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Literature summary extracted from
- Purification and biochemical characterization of a poly(ADP-ribose) polymerase-like enzyme from the thermophilic archaeon Sulfolobus solfataricus. (1998), Biochem. J., 335, 441-447.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.2.30 | 0.154 | - |
NAD+ | - |
Saccharolobus solfataricus |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.2.30 | Saccharolobus solfataricus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.2.30 | - |
Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.4.2.30 | 83 | - |
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.2.30 | NAD+ + (ADP-D-ribosyl)n-acceptor | - |
Saccharolobus solfataricus | nicotinamide + (ADP-D-ribosyl)n+1-acceptor | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.2.30 | ? | x * 54000-55000, SDS-PAGE | Saccharolobus solfataricus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.4.2.30 | PARPss | - |
Saccharolobus solfataricus |
| 2.4.2.30 | poly(ADP-ribose) polymerase-like enzyme | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.2.30 | 80 | - |
- |
Saccharolobus solfataricus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.2.30 | 80 | - |
half-life: 204 min | Saccharolobus solfataricus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.2.30 | 0.04 | - |
NAD+ | - |
Saccharolobus solfataricus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.2.30 | 8 | - |
- |
Saccharolobus solfataricus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.2.30 | 7 | 9.5 | pH 7.0: about 75% of maximal activity, pH 9.5: about 70% of maximal activity | Saccharolobus solfataricus |
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