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Literature summary extracted from

  • Dewanti, A.R.; Xu, Y.; Mitra, B.
    Esters of mandelic acid as substrates for (S)-mandelate dehydrogenase from Pseudomonas putida: implications for the reaction mechanism (2004), Biochemistry, 43, 1883-1890.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.99.31 R277G reduced activity compared to the wild type enzyme, the mutation at Arg277 has no influence on the binding affinity of ester substrates Pseudomonas putida
1.1.99.31 R277K reduced activity compared to the wild type enzyme, the mutation at Arg277 has no influence on the binding affinity of ester substrates Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.31 (S)-1-phenyl-1,2-ethanediol weak inhibitor Pseudomonas putida
1.1.99.31 (S)-1-phenyl-2,2,2-trifluoroethanol competitive inhibitor Pseudomonas putida
1.1.99.31 (S)-1-phenyl-2-propen-1-ol
-
 Pseudomonas putida
1.1.99.31 (S)-1-phenyl-2-propyn-1-ol
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.31 0.12
-
 (S)-Mandelate wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
1.1.99.31 2.7
-
 ethyl-(S)-mandelate mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
1.1.99.31 2.9
-
 ethyl-(S)-mandelate wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
1.1.99.31 3.7
-
 ethyl-(S)-mandelate mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
1.1.99.31 5.6
-
 (S)-Mandelate mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
1.1.99.31 7.4
-
 methyl-(S)-mandelate mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
1.1.99.31 10.3
-
 methyl-(S)-mandelate mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
1.1.99.31 11.3
-
 methyl-(S)-mandelate wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
1.1.99.31 17
-
 (S)-Mandelate mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.31 Pseudomonas putida P20932
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.31
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.31 (S)-mandelate + 2 ferricyanide
-
 Pseudomonas putida 2-oxo-2-phenylacetate + 2 ferrocyanide + 2 H+
-
 ?
1.1.99.31 ethyl-(S)-mandelate + acceptor
-
 Pseudomonas putida ?
-
 ?
1.1.99.31 methyl-(S)-mandelate + acceptor
-
 Pseudomonas putida ?
-
 ?
1.1.99.31 additional information (S)-mandelamide, (S)-1-phenyl-2-propen-1-ol, (S)-1-phenyl-1,2-ethanediol, (S)-1-phenyl-2-propyn-1-ol, (R,S)-pantoyllactone, and (S)-1-phenyl-2,2,2-trifluoroethanol are no substrates Pseudomonas putida ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.31 MDH
-
 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.31 0.23
-
 (S)-Mandelate mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
1.1.99.31 0.3
-
 ethyl-(S)-mandelate mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
1.1.99.31 2.3
-
 methyl-(S)-mandelate mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
1.1.99.31 2.5
-
 methyl-(S)-mandelate mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
1.1.99.31 2.6
-
 ethyl-(S)-mandelate mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
1.1.99.31 66
-
 (S)-Mandelate mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
1.1.99.31 151
-
 methyl-(S)-mandelate wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
1.1.99.31 204
-
 ethyl-(S)-mandelate wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
1.1.99.31 360
-
 (S)-Mandelate wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.31 FMN dependent Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.99.31 2.6
-
 (S)-1-phenyl-2,2,2-trifluoroethanol wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
1.1.99.31 53.8
-
 (S)-1-phenyl-1,2-ethanediol wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida