Literature summary extracted from
Gogliettino, M.A.; Tanfani, F.; Scire, A.; Ursby, T.; Adinolfi, B.S.; Cacciamani, T.; De Vendittis, E.
The role of Tyr41 and His155 in the functional properties of superoxide dismutase from the archaeon Sulfolobus solfataricus (2004), Biochemistry, 43, 2199-2208.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.15.1.1 |
expression of wild-type and mutant enzymes in Escherichia coli strain JM109(DE3) |
Saccharolobus solfataricus |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.15.1.1 |
purified mutant enzyme Y41F, hanging drop vapor diffusion method, 21°C, 1:1 mix of the reservoir solution containing 8% PEG 8000, 0.1 M Tris-HCl, pH 8.5, and the protein solution containing 1.45 mg/mL Y41F, 20 mM Tris-HCl, pH 7.8, and 1% glycerol, X-ray diffraction structure determination and analysis |
Saccharolobus solfataricus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.15.1.1 |
H155Q |
site-directed mutagenesis, the mutant shows a a slightly lower iron content, reduced heat stability, and a 2fold reduced activity compared to the wild-type enzyme |
Saccharolobus solfataricus |
1.15.1.1 |
Y41F |
site-directed mutagenesis, the mutant shows a a slightly lower iron content and a 17fold reduced activity compared to the wild-type enzyme, the mutant shows an uninterrupted hydrogen bond network |
Saccharolobus solfataricus |
General Stability
EC Number |
General Stability |
Organism |
---|
1.15.1.1 |
SDS decreases the stability of SOD and accelerates enzyme inactivation |
Saccharolobus solfataricus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.15.1.1 |
NaN3 |
inhibits the enzyme mutants Y41F and H155Q, but not the wild-type enzyme |
Saccharolobus solfataricus |
|
1.15.1.1 |
PMSF |
irreversible inhibition by binding to active site Tyr41 |
Saccharolobus solfataricus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.15.1.1 |
Fe2+ |
Fe-type SOD |
Saccharolobus solfataricus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.15.1.1 |
24000 |
- |
4 * 24000, wild-type and mutant enzymes, SDS-PAGE |
Saccharolobus solfataricus |
1.15.1.1 |
87900 |
- |
wild-type and mutant enzymes, gel filtration |
Saccharolobus solfataricus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.15.1.1 |
Saccharolobus solfataricus |
- |
- |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.15.1.1 |
387 |
- |
purified mutant Y41F |
Saccharolobus solfataricus |
1.15.1.1 |
3980 |
- |
purified mutant H155Q |
Saccharolobus solfataricus |
1.15.1.1 |
6720 |
- |
purified wild-type enzyme |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.15.1.1 |
additional information |
covalent modification of the conserved Tyr41 in the active site, Tyr41 and His155 are involved in catalysis, hydrogen bond network including three solvent molecules connecting the iron-ligating hydroxide ion via H155 with F41 and H37, Y41 and H155 are important for the structural and functional properties of SOD, overview |
Saccharolobus solfataricus |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.15.1.1 |
tetramer |
4 * 24000, wild-type and mutant enzymes, SDS-PAGE |
Saccharolobus solfataricus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.15.1.1 |
SOD |
- |
Saccharolobus solfataricus |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
1.15.1.1 |
additional information |
- |
- |
Saccharolobus solfataricus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.15.1.1 |
5.5 |
7.4 |
assay at |
Saccharolobus solfataricus |
pI Value
EC Number |
Organism |
Comment |
pI Value Maximum |
pI Value |
---|
1.15.1.1 |
Saccharolobus solfataricus |
isoelectric focusing |
- |
8.4 |