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Literature summary extracted from

  • Gogliettino, M.A.; Tanfani, F.; Scire, A.; Ursby, T.; Adinolfi, B.S.; Cacciamani, T.; De Vendittis, E.
    The role of Tyr41 and His155 in the functional properties of superoxide dismutase from the archaeon Sulfolobus solfataricus (2004), Biochemistry, 43, 2199-2208.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 expression of wild-type and mutant enzymes in Escherichia coli strain JM109(DE3) Saccharolobus solfataricus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.15.1.1 purified mutant enzyme Y41F, hanging drop vapor diffusion method, 21°C, 1:1 mix of the reservoir solution containing 8% PEG 8000, 0.1 M Tris-HCl, pH 8.5, and the protein solution containing 1.45 mg/mL Y41F, 20 mM Tris-HCl, pH 7.8, and 1% glycerol, X-ray diffraction structure determination and analysis Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
1.15.1.1 H155Q site-directed mutagenesis, the mutant shows a a slightly lower iron content, reduced heat stability, and a 2fold reduced activity compared to the wild-type enzyme Saccharolobus solfataricus
1.15.1.1 Y41F site-directed mutagenesis, the mutant shows a a slightly lower iron content and a 17fold reduced activity compared to the wild-type enzyme, the mutant shows an uninterrupted hydrogen bond network Saccharolobus solfataricus

General Stability

EC Number General Stability Organism
1.15.1.1 SDS decreases the stability of SOD and accelerates enzyme inactivation Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 NaN3 inhibits the enzyme mutants Y41F and H155Q, but not the wild-type enzyme Saccharolobus solfataricus
1.15.1.1 PMSF irreversible inhibition by binding to active site Tyr41 Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Fe2+ Fe-type SOD Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 24000
-
 4 * 24000, wild-type and mutant enzymes, SDS-PAGE Saccharolobus solfataricus
1.15.1.1 87900
-
 wild-type and mutant enzymes, gel filtration Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Saccharolobus solfataricus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 387
-
 purified mutant Y41F Saccharolobus solfataricus
1.15.1.1 3980
-
 purified mutant H155Q Saccharolobus solfataricus
1.15.1.1 6720
-
 purified wild-type enzyme Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 additional information covalent modification of the conserved Tyr41 in the active site, Tyr41 and His155 are involved in catalysis, hydrogen bond network including three solvent molecules connecting the iron-ligating hydroxide ion via H155 with F41 and H37, Y41 and H155 are important for the structural and functional properties of SOD, overview Saccharolobus solfataricus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 tetramer 4 * 24000, wild-type and mutant enzymes, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 SOD
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 additional information
-
-
 Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 5.5 7.4 assay at Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.15.1.1 Saccharolobus solfataricus isoelectric focusing
-
 8.4