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Literature summary extracted from

  • Fromant, M.; Schmitt, E.; Mechulam, Y.; Lazennec, C.; Plateau, P.; Blanquet, S.
    Crystal structure at 1.8 A resolution and identification of active site residues of Sulfolobus solfataricus peptidyl-tRNA hydrolase (2005), Biochemistry, 44, 4294-4301.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.29 pth2 gene, expression in Escherichia coli Saccharolobus solfataricus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.29 purified recombinant enzyme, hanging drop vapour diffusion method, 24°C, 0.0027 ml of 1.3 mg/ml protein in 20 mM Tris-HCl, pH 7.0, 0.1 mM EDTA, and 10 mM 2-mercaptoethanol are mixed with 0.0007 ml of 11 mg/ml tRNAfMet solution and 0.002 ml of reservoir solution containing 0.8 M LiSO4 and 1.6% PEG 8000, crystallization of tRNA free crystals within a few days, X-ray diffraction structure determination and analysis of native and HgBr2-containing crystals at 1.8-3.0 A resolution, modeling Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.29 Saccharolobus solfataricus Q980V1 gene pth2
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.29 N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA residues K18, D86, and T90 are essential for catalytic activity, they are located in the N-part of alpha1 and in the beta3-beta4 loop. K18 and D86, which form a salt bridge, might play a role in the catalysis thanks to their acid and basic functions, whereas the OH group of T90 could act as a nucleophile Saccharolobus solfataricus
a

Subunits

EC Number Subunits Comment Organism
3.1.1.29 dimer crystal structure analysis, three-dimensional structure, each protomer is made of a mixed five-stranded beta-sheet surrounded by two groups of two alpha-helices, the dimer interface is mainly formed by van der Waals interactions between hydrophobic residues belonging to the two N-terminal R1 helices contributed by two protomers, overview Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.29 peptidyl-tRNA hydrolase
-
 Saccharolobus solfataricus
3.1.1.29 suPth2
-
 Saccharolobus solfataricus