Literature summary extracted from
Fromant, M.; Schmitt, E.; Mechulam, Y.; Lazennec, C.; Plateau, P.; Blanquet, S.
Crystal structure at 1.8 A resolution and identification of active site residues of Sulfolobus solfataricus peptidyl-tRNA hydrolase (2005), Biochemistry, 44, 4294-4301.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.1.29 |
pth2 gene, expression in Escherichia coli |
Saccharolobus solfataricus |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.1.1.29 |
purified recombinant enzyme, hanging drop vapour diffusion method, 24°C, 0.0027 ml of 1.3 mg/ml protein in 20 mM Tris-HCl, pH 7.0, 0.1 mM EDTA, and 10 mM 2-mercaptoethanol are mixed with 0.0007 ml of 11 mg/ml tRNAfMet solution and 0.002 ml of reservoir solution containing 0.8 M LiSO4 and 1.6% PEG 8000, crystallization of tRNA free crystals within a few days, X-ray diffraction structure determination and analysis of native and HgBr2-containing crystals at 1.8-3.0 A resolution, modeling |
Saccharolobus solfataricus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.1.29 |
Saccharolobus solfataricus |
Q980V1 |
gene pth2 |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
3.1.1.29 |
N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA |
residues K18, D86, and T90 are essential for catalytic activity, they are located in the N-part of alpha1 and in the beta3-beta4 loop. K18 and D86, which form a salt bridge, might play a role in the catalysis thanks to their acid and basic functions, whereas the OH group of T90 could act as a nucleophile |
Saccharolobus solfataricus |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.1.1.29 |
dimer |
crystal structure analysis, three-dimensional structure, each protomer is made of a mixed five-stranded beta-sheet surrounded by two groups of two alpha-helices, the dimer interface is mainly formed by van der Waals interactions between hydrophobic residues belonging to the two N-terminal R1 helices contributed by two protomers, overview |
Saccharolobus solfataricus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.1.29 |
peptidyl-tRNA hydrolase |
- |
Saccharolobus solfataricus |
3.1.1.29 |
suPth2 |
- |
Saccharolobus solfataricus |