Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sullivan, S.M.; Holyoak, T.
    Structures of rat cytosolic PEPCK: insight into the mechanism of phosphorylation and decarboxylation of oxaloacetic acid (2007), Biochemistry, 46, 10078-10088.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.32 expressed in Escherichia coli BL21 cells Rattus norvegicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.32 hanging drop vapour diffusion method, at 25 ?C in 0.1 M HEPES (pH 7.4) and 12-30% PEG 3350, creating PEPCK-Mn2+, PEPCK-Mn2+-oxaloacetate, PEPCK-Mn2+-oxaloacetate-Mn2+GDP, and PEPCKMn2+-Mn2+GTP crystals Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.32 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.32 Mn2+ required Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.32 Rattus norvegicus P07379
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.32 glutathione-Uniflow resin column chromatography and P6DG column chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.32 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.32 GTP + oxaloacetate
-
 Rattus norvegicus GDP + phosphoenolpyruvate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.32 PEPCK
-
 Rattus norvegicus