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Literature summary extracted from

  • Ruocco, M.R.; Ruggiero, A.; Masullo, L.; Arcari, P.; Masullo, M.
    A 35 kDa NAD(P)H oxidase previously isolated from the archaeon Sulfolobus solfataricus is instead a thioredoxin reductase. (2004), Biochimie, 86, 883-892.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.9 expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.9 C147A significantly reduced activity, decrease in FAD content Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.9 auranofin
-
 Saccharolobus solfataricus
1.8.1.9 azelaic acid
-
 Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.9 0.0017
-
 NADPH pH 5.5, 60°C Saccharolobus solfataricus
1.8.1.9 0.0441
-
 thioredoxin pH 5.5, 60°C Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 35000
-
 2 * 35000, SDS-PAGE Saccharolobus solfataricus
1.8.1.9 70000
-
 gel filtration Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Saccharolobus solfataricus Q97W27 enzyme shows additionally NADH oxidase activity
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
-
 Saccharolobus solfataricus 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 additional information enzyme shows additionally NADH oxidase activity Saccharolobus solfataricus ?
-
 ?
1.8.1.9 thioredoxin + NADP+
-
 Saccharolobus solfataricus thioredoxin disulfide + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.9 dimer 2 * 35000, SDS-PAGE Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.9 0.61
-
 NADPH pH 5.5, 60°C Saccharolobus solfataricus
1.8.1.9 1.3
-
 thioredoxin pH 5.5, 60°C Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD wild-type, 0.7 mol per mol of subunit, mutant C147A, 0.3 mol per mol of subunit Saccharolobus solfataricus
1.8.1.9 NADP+
-
 Saccharolobus solfataricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.1.9 0.013
-
 auranofin pH 5.5, 60°C Saccharolobus solfataricus
1.8.1.9 0.054
-
 azelaic acid pH 5.5, 60°C Saccharolobus solfataricus