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Literature summary extracted from

  • Bai, B.; Xie, J.; Yan, J.; Wang, H.; Hu, C.
    A high throughput screening approach to identify isocitrate lyase inhibitors from traditional Chinese medicine sources (2006), Drug Dev. Res., 67, 818-823.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.1 expressed in Escherichia coli strain JM 109 Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.1 itaconic anhydride
-
 Mycobacterium tuberculosis
4.1.3.1 XHD-1 extract of Illicium verum Mycobacterium tuberculosis
4.1.3.1 XHD-2 extract of Zingiber officinale Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.159
-
 isocitrate in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1 Ni-Sepharose chromatography Mycobacterium tuberculosis

Storage Stability

EC Number Storage Stability Organism
4.1.3.1 4°C, 50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH 8.0, 2 months, no loss of activity Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 isocitrate
-
 Mycobacterium tuberculosis succinate + glyoxylate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.3.1 ICL
-
 Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.1.3.1 0.0033
-
 in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C Mycobacterium tuberculosis itaconic anhydride
4.1.3.1 0.0182
-
 in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C Mycobacterium tuberculosis XHD-2
4.1.3.1 0.0477
-
 in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C Mycobacterium tuberculosis XHD-1