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Literature summary extracted from

  • Schepers, B.; Thiemann, V.; Antranikian, G.
    Characterization of a novel glucoamylase from the thermoacidophilic archaeon Picrophilus torridus heterologously expressed in E. coli (2006), Eng. Life Sci., 6, 311-317.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.3 phylogenetic tree, expression of the intracellular enzyme in Escherichia coli Picrophilus torridus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 1.9
-
maltotriose pH 5.0, 50°C, recombinant enzyme Picrophilus torridus
3.2.1.3 4.9
-
maltose pH 5.0, 50°C, recombinant enzyme Picrophilus torridus
3.2.1.3 9.9
-
maltotetraose pH 5.0, 50°C, recombinant enzyme Picrophilus torridus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.3 extracellular
-
Picrophilus torridus
-
-
3.2.1.3 intracellular
-
Picrophilus torridus 5622
-
3.2.1.3 additional information the extracellular enzyme contains a signal sequence Picrophilus torridus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 73000
-
4 * 73000, intracellular enzyme, SDS-PAGE Picrophilus torridus
3.2.1.3 92000
-
x * 92000, extracellular enzyme Picrophilus torridus
3.2.1.3 292000
-
recombinant intracellular enzyme, native PAGE Picrophilus torridus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Picrophilus torridus Q6KYX5
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.3 additional information the extracellular enzyme contains a signal sequence Picrophilus torridus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 recombinant intracellular enzyme 37fold from Escherichia coli by heat treatment at 70°C for 30 min, anion exchange chromatography, and gel filtration to homogeneity Picrophilus torridus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.3 cell culture optimal growth at pH 0.7 and 60 °C Picrophilus torridus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 amylopectin + H2O potato amylopectin Picrophilus torridus D-glucose + ?
-
?
3.2.1.3 amylose + H2O potato amylose type III Picrophilus torridus D-glucose + ?
-
?
3.2.1.3 dextrin + 6 H2O
-
Picrophilus torridus 7 D-glucose
-
?
3.2.1.3 maltose + H2O
-
Picrophilus torridus 2 D-glucose
-
?
3.2.1.3 maltotetraose + H2O
-
Picrophilus torridus maltotriose + D-glucose
-
?
3.2.1.3 maltotriose + H2O
-
Picrophilus torridus maltose + D-glucose
-
?
3.2.1.3 additional information the intracellular enzyme shows a unique substrate specificity compared to already known glucoamylases, in addition to the hydrolysis of branched and linear alpha-glucans, the purified enzyme preferentially attacks maltotriose, overview, oligossaccharides Dp2, Dp4, Dp5, Dp6, and Dp7 as well as isomaltose, panose, and isopanose are poor substrates Picrophilus torridus ?
-
?
3.2.1.3 starch + H2O
-
Picrophilus torridus D-glucose + ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 ? x * 92000, extracellular enzyme Picrophilus torridus
3.2.1.3 tetramer 4 * 73000, intracellular enzyme, SDS-PAGE Picrophilus torridus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 glucoamylase
-
Picrophilus torridus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 50
-
intracellular enzyme Picrophilus torridus
3.2.1.3 90
-
extracellular enzyme Picrophilus torridus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.3 30 65 intracellular enzyme Picrophilus torridus
3.2.1.3 50 100 extracellular enzyme Picrophilus torridus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 50
-
purified recombinant intracellular enzyme, 48 h, stable Picrophilus torridus
3.2.1.3 55
-
purified recombinant intracellular enzyme, half-life is 8 h Picrophilus torridus
3.2.1.3 60
-
purified recombinant intracellular enzyme, half-life is 1 h Picrophilus torridus
3.2.1.3 65
-
purified recombinant intracellular enzyme, inctivation within 5 min Picrophilus torridus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 2
-
extracellular enzyme Picrophilus torridus
3.2.1.3 5
-
intracellular enzyme Picrophilus torridus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.3
-
4 extracellular enzyme Picrophilus torridus
3.2.1.3 4.5 6.5 intracellular enzyme Picrophilus torridus