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Literature summary extracted from

  • Lee, J.; Yoon, S.; Nam, S.; Moon, Y.; Moon, Y.; Kim, D.
    Molecular cloning of a gene encoding the sucrose phosphorylase from Leuconostoc mesenteroides B-1149 and the expression in Escherichia coli (2006), Enzyme Microb. Technol., 39, 612-620.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.7 expression in Escherichia coli Leuconostoc mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.7 6.3
-
 sucrose
-
 Leuconostoc mesenteroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.7 56100
-
 x * 56100, calculation from sequence Leuconostoc mesenteroides
2.4.1.7 60000
-
 x * 60000, SDS-PAGE Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.7 Leuconostoc mesenteroides Q14EH6
-
-
2.4.1.7 Leuconostoc mesenteroides B-1149 Q14EH6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.7
-
 Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.7 1.49
-
-
 Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.7 sucrose + acarbose transglucosylation Leuconostoc mesenteroides ?
-
 ?
2.4.1.7 sucrose + cellobiose transglucosylation Leuconostoc mesenteroides ?
-
 ?
2.4.1.7 sucrose + cellobiose transglucosylation Leuconostoc mesenteroides B-1149 ?
-
 ?
2.4.1.7 sucrose + D-arabinose transglucosylation Leuconostoc mesenteroides ?
-
 ?
2.4.1.7 sucrose + D-arabinose transglucosylation Leuconostoc mesenteroides B-1149 ?
-
 ?
2.4.1.7 sucrose + D-fructose transglucosylation Leuconostoc mesenteroides ?
-
 ?
2.4.1.7 sucrose + D-glucose transglucosylation Leuconostoc mesenteroides ?
-
 ?
2.4.1.7 sucrose + erythritol transglucosylation Leuconostoc mesenteroides ?
-
 ?
2.4.1.7 sucrose + galactose transglucosylation Leuconostoc mesenteroides ?
-
 ?
2.4.1.7 sucrose + isomaltotriose transglucosylation Leuconostoc mesenteroides ?
-
 ?
2.4.1.7 sucrose + lactose transglucosylation Leuconostoc mesenteroides ?
-
 ?
2.4.1.7 sucrose + lactose transglucosylation Leuconostoc mesenteroides B-1149 ?
-
 ?
2.4.1.7 sucrose + maltose transglucosylation Leuconostoc mesenteroides ?
-
 ?
2.4.1.7 sucrose + maltose transglucosylation Leuconostoc mesenteroides B-1149 ?
-
 ?
2.4.1.7 sucrose + maltotriose transglucosylation Leuconostoc mesenteroides ?
-
 ?
2.4.1.7 sucrose + maltotriose transglucosylation Leuconostoc mesenteroides B-1149 ?
-
 ?
2.4.1.7 sucrose + palatinose transglucosylation Leuconostoc mesenteroides ?
-
 ?
2.4.1.7 sucrose + rhamnose transglucosylation Leuconostoc mesenteroides ?
-
 ?
2.4.1.7 sucrose + salicin transglucosylation Leuconostoc mesenteroides ?
-
 ?
2.4.1.7 sucrose + sorbitol transglucosylation Leuconostoc mesenteroides ?
-
 ?
2.4.1.7 sucrose + xylitol transglucosylation Leuconostoc mesenteroides ?
-
 ?
2.4.1.7 sucrose + xylose transglucosylation Leuconostoc mesenteroides ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.7 ? x * 60000, SDS-PAGE Leuconostoc mesenteroides
2.4.1.7 ? x * 56100, calculation from sequence Leuconostoc mesenteroides

Synonyms

EC Number Synonyms Comment Organism
2.4.1.7 1149SPase
-
 Leuconostoc mesenteroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.7 20 50
-
 Leuconostoc mesenteroides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.7 20 75 20°C-50°C: maximal activity, 75°C: about 50% of maximal activity Leuconostoc mesenteroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.7 50
-
 30 min, enzyme retains over 70% of the initial activity Leuconostoc mesenteroides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.7 1.59
-
 sucrose
-
 Leuconostoc mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.7 6 7.5
-
 Leuconostoc mesenteroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.7 5 8 pH 5.0: about 40% of maximal activity, pH 8.0: about 20% of maximal activity Leuconostoc mesenteroides