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Literature summary extracted from

  • De Vendittis, E.; Adinolfi, B.S.; Amatruda, M.R.; Raimo, G.; Masullo, M.; Bocchini, V.
    The A26G replacement in the consensus sequence A-X-X-X-X-G-K-[T,S] of the guanine nucleotide binding site activates the intrinsic GTPase of the elongation factor 2 from the archaeon Sulfolobus solfataricus (1999), Eur. J. Biochem., 262, 600-605.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.5.1 ethylene glycol
-
 Saccharolobus solfataricus
3.6.5.1 additional information GTPase activity is stimulated by ribosomes Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.5.1 into the pT7-7 vector for expession in Escherichia coli JM109DE3 cells Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
3.6.5.1 A26G mutant, rate of poly(U)-directed poly(Phe) synthesis and the ribosome-dependent GTPase activity are decreased, the catalytic efficiency of the intrinsic SsEF-2 GTPase triggered by ethylene glycol is enhanced Saccharolobus solfataricus
3.6.5.3 A26G converts the guanine nucleotide binding consensus sequences A-X-X-X-X-G-K-[T,S] of the elongation factor EF-2 into the corresponding G-X-X-X-X-G-K-[T,S] motif which is present in all the other GTP-binding proteins. In the mutant, the rate of poly(U)-directed poly(Phe) synthesis and the ribosome-dependent GTPase activity of A26GSsEF-2 are decreased. A26G substitution enhances the catalytic efficiency of the intrinsic SsEF-2 GTPase triggered by ethylene glycol and decreases the affinity for GDP Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.5.1 GDP competitive inhibition Saccharolobus solfataricus
3.6.5.3 GDP
-
 Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.5.1 0.0008
-
 GTP A26GSsEF-2, 40% glycol Saccharolobus solfataricus
3.6.5.1 0.001
-
 GTP SsEF-2, 40% glycol Saccharolobus solfataricus
3.6.5.1 0.0055
-
 GTP A26GSsEF-2, 10% glycol Saccharolobus solfataricus
3.6.5.1 0.0096
-
 GTP SsEF-2, 10% glycol Saccharolobus solfataricus
3.6.5.1 0.0194
-
 GTP A26GSsEF-2, 0% glycol Saccharolobus solfataricus
3.6.5.3 0.0008
-
 GTP mutant A26G, pH 7.5, 60°C, presence of 40% ethylene glycol Saccharolobus solfataricus
3.6.5.3 0.001
-
 GTP wild-type, pH 7.5, 60°C, presence of 40% ethylene glycol Saccharolobus solfataricus
3.6.5.3 0.0055
-
 GTP mutant A26G, pH 7.5, 60°C, presence of 10% ethylene glycol Saccharolobus solfataricus
3.6.5.3 0.0096
-
 GTP wild-type, pH 7.5, 60°C, presence of 10% ethylene glycol Saccharolobus solfataricus
3.6.5.3 0.0194
-
 GTP mutant A26G, pH 7.5, 60°C Saccharolobus solfataricus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.5.1 ribosome
-
 Saccharolobus solfataricus 5840
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.5.1 GTP + H2O Saccharolobus solfataricus
-
 GDP + phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.6.5.1 Saccharolobus solfataricus
-
-
-
3.6.5.3 Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.5.1 GTP + H2O
-
 Saccharolobus solfataricus GDP + phosphate
-
 ir
3.6.5.3 GTP + H2O
-
 Saccharolobus solfataricus GDP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.5.1 EF-2
-
 Saccharolobus solfataricus
3.6.5.1 EF-G
-
 Saccharolobus solfataricus
3.6.5.1 elongation factor 2
-
 Saccharolobus solfataricus
3.6.5.1 heterotrimeric G-protein GTPase
-
 Saccharolobus solfataricus
3.6.5.1 SsEF-2
-
 Saccharolobus solfataricus
3.6.5.3 elongation factor 2
-
 Saccharolobus solfataricus
3.6.5.3 SsEF-2
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.5.1 60
-
 activity assay Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.5.1 0.2
-
 GTP SsEF-2, 10% glycol Saccharolobus solfataricus
3.6.5.1 1.9
-
 GTP SsEF-2, 40% glycol Saccharolobus solfataricus
3.6.5.1 7.2
-
 GTP A26GSsEF-2, 10% glycol Saccharolobus solfataricus
3.6.5.1 8
-
 GTP A26GSsEF-2, 0% glycol Saccharolobus solfataricus
3.6.5.1 9.9
-
 GTP A26GSsEF-2, 40% glycol Saccharolobus solfataricus
3.6.5.3 0.0033
-
 GTP wild-type, pH 7.5, 60°C, presence of 10% ethylene glycol Saccharolobus solfataricus
3.6.5.3 0.032
-
 GTP wild-type, pH 7.5, 60°C, presence of 40% ethylene glycol Saccharolobus solfataricus
3.6.5.3 0.12
-
 GTP mutant A26G, pH 7.5, 60°C, presence of 10% ethylene glycol Saccharolobus solfataricus
3.6.5.3 0.133
-
 GTP mutant A26G, pH 7.5, 60°C Saccharolobus solfataricus
3.6.5.3 0.165
-
 GTP mutant A26G, pH 7.5, 60°C, presence of 40% ethylene glycol Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.5.1 7.5
-
 activity assay Saccharolobus solfataricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.5.1 0.0003
-
 GDP SsEF-2, 40% glycol Saccharolobus solfataricus
3.6.5.1 0.0005
-
 GDP A26GSsEF-2, 40% glycol Saccharolobus solfataricus
3.6.5.1 0.0074
-
 GDP A26GSsEF-2, 0% glycol Saccharolobus solfataricus
3.6.5.3 0.0003
-
 GDP wild-type, pH 7.5, 60°C, presence of 40% ethylene glycol Saccharolobus solfataricus
3.6.5.3 0.0005
-
 GDP mutant A26G, pH 7.5, 60°C, presence of 40% ethylene glycol Saccharolobus solfataricus
3.6.5.3 0.0074
-
 GDP mutant A26G, pH 7.5, 60°C Saccharolobus solfataricus