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Literature summary extracted from

  • De Vendittis, E.; Ursby, T.; Rullo, R.; Gogliettino, M.A.; Masullo, M.; Bocchini, V.
    Phenylmethanesulfonyl fluoride inactivates an archaeal superoxide dismutase by chemical modification of a specific tyrosine residue. Cloning, sequencing and expression of the gene coding for Sulfolobus solfataricus superoxide dismutase (2001), Eur. J. Biochem., 268, 1794-1801.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 gene sod, cloning from genomic DNA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109(DE3) Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 phenylmethanesulfonyl fluoride irreversible inactivation by attachment of a molecule phenylmethanesulfonyl fluoride to the active site Tyr41 reinforcing the heat stability of the enzyme, overview Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Fe2+ Fe-type SOD Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 24000
-
 4 * 24000, recombinant enzyme, SDS-PAGE Saccharolobus solfataricus
1.15.1.1 88000
-
 recombinant enzyme, gel filtration Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Saccharolobus solfataricus P80857
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 native enzyme, and recombinant enzyme from Escherichia coli strain JM109(DE3) Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 additional information native and recombinant enzyme ossess a covalent modification of the conserved Tyr41 in the active site, Tyr41 plays an important role in the enzyme activity and the maintenance of the structural architecture of SOD, overview Saccharolobus solfataricus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 tetramer 4 * 24000, recombinant enzyme, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 SOD
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 additional information
-
 phenylmethanesulfonyl fluoride attachment to the active site Tyr41 increases the heat stability of the enzyme, overview Saccharolobus solfataricus