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Literature summary extracted from

  • Shima, S.; Matsui, H.; Tahara, S.; Imai, R.
    Biochemical characterization of rice trehalose-6-phosphate phosphatases supports distinctive functions of these plant enzymes (2007), FEBS J., 274, 1192-1201.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.12 expressed in Escherichia coli BL21 cells Oryza sativa Japonica Group
3.1.3.12 expressed in Escherichia coli BL21 cells Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.12 0.0921
-
 trehalose 6-phosphate in 50mM Tris /HCl buffer with 2mM MgCl2, at 37°C Oryza sativa
3.1.3.12 0.186
-
 trehalose 6-phosphate in 50 mM Tris/HCl buffer with 2 mM MgCl2, at 37°C Oryza sativa Japonica Group

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.12 42600
-
 deduced from the nucleotide sequence Oryza sativa Japonica Group
3.1.3.12 45000
-
 SDS-PAGE Oryza sativa Japonica Group

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.12 Oryza sativa Q75WV3 cultivar Yukihikari
-
3.1.3.12 Oryza sativa Japonica Group Q9FWQ2 cultivar Yukihikari
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.12 glutathione-Sepharose 4B affinity column chromatography Oryza sativa Japonica Group
3.1.3.12 glutathione-Sepharose 4B affinity column chromatography Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.12 root
-
 Oryza sativa Japonica Group
-
3.1.3.12 root
-
 Oryza sativa
-
3.1.3.12 shoot
-
 Oryza sativa Japonica Group
-
3.1.3.12 shoot
-
 Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.12 additional information almost no activity with glucose 1-phosphate, glucose 6-phosphate, galactose 6-phosphate, mannose 1-phosphate, mannose 6-phosphate, fructose 1-phosphate, fructose 6-phosphate, sucrose 6-phosphate, lactose 1-phosphate, and ribose 5-phosphate Oryza sativa Japonica Group ?
-
 ?
3.1.3.12 additional information almost no activity with glucose 1-phosphate, glucose 6-phosphate, galactose 6-phosphate, mannose 1-phosphate, mannose 6-phosphate, fructose 1-phosphate, fructose 6-phosphate, sucrose 6-phosphate, lactose 1-phosphate, and ribose 5-phosphate Oryza sativa ?
-
 ?
3.1.3.12 trehalose 6-phosphate + H2O
-
 Oryza sativa Japonica Group trehalose + phosphate
-
 ?
3.1.3.12 trehalose 6-phosphate + H2O
-
 Oryza sativa trehalose + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.12 Tpp1
-
 Oryza sativa
3.1.3.12 TPP2
-
 Oryza sativa Japonica Group
3.1.3.12 trehalose-6-phosphate phosphatase
-
 Oryza sativa Japonica Group
3.1.3.12 trehalose-6-phosphate phosphatase
-
 Oryza sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.12 50
-
 almost completely loses activity when subjected to heat treatment at 50°C for 4 min Oryza sativa Japonica Group
3.1.3.12 50
-
 almost completely loses activity when subjected to heat treatment at 50°C for 4 min Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.12 0.03 0.55 trehalose 6-phosphate in 50mM Tris /HCl buffer with 2mM MgCl2, at 37°C Oryza sativa
3.1.3.12 6.52
-
 trehalose 6-phosphate in 50mM Tris /HCl buffer with 2mM MgCl2, at 37°C Oryza sativa
3.1.3.12 13.4
-
 trehalose 6-phosphate in 50 mM Tris/HCl buffer with 2 mM MgCl2, at 37°C Oryza sativa Japonica Group

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.12 6.5
-
-
 Oryza sativa Japonica Group
3.1.3.12 7
-
-
 Oryza sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.12 5.5 9
-
 Oryza sativa Japonica Group
3.1.3.12 5.5 9
-
 Oryza sativa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.12 5.5 9 almost no activity at pH 5.5 or 9.0 Oryza sativa Japonica Group
3.1.3.12 5.5 9 almost no activity at pH 5.5 or 9.0 Oryza sativa