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Literature summary extracted from
- Mechanism of dihydroneopterin aldolase. NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes (2007), FEBS J., 274, 2240-2252.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.2.25 | expressed in Escherichia coli BL21(DE3) cells | Staphylococcus aureus |
| 4.1.2.25 | expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.2.25 | 10 | - |
glycoaldehyde | apparent value | Staphylococcus aureus |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.2.25 | Escherichia coli | - |
- |
- |
| 4.1.2.25 | Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.2.25 | Ni-nitrilotriacetate column chromatography, DEAE-cellulose column chromatography and Bio-Gel A-0.5 m gel filtration | Staphylococcus aureus |
| 4.1.2.25 | Ni-nitrilotriacetate column chromatography, DEAE-cellulose column chromatography and Bio-Gel A-0.5 m gel filtration | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.25 | 7,8-dihydroneopterin | - |
Staphylococcus aureus | 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde | - |
r | |
| 4.1.2.25 | 7,8-dihydroneopterin | - |
Escherichia coli | 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.2.25 | DHNA | DHNA catalyzes both the cleavage of 7,8-dihydroneopterin to form 6-hydroxymethyl-7,8-dihydropterin and glycolaldehyde and the epimerization of 7,8-dihydroneopterin to form 7,8-dihydro-l-monapterin | Staphylococcus aureus |
| 4.1.2.25 | DHNA | DHNA catalyzes both the cleavage of 7,8-dihydroneopterin to form 6-hydroxymethyl-7,8-dihydropterin and glycolaldehyde and the epimerization of 7,8-dihydroneopterin to form 7,8-dihydro-l-monapterin | Escherichia coli |
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Release 2023.1 (January 2023)
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