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Literature summary extracted from

  • Desmet, T.; Cantaert, T.; Gualfetti, P.; Nerinckx, W.; Gross, L.; Mitchinson, C.; Piens, K.
    An investigation of the substrate specificity of the xyloglucanase Cel74A from Hypocrea jecorina (2007), FEBS J., 274, 356-363.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.151 additional information
-
 additional information Km values are 0.8 mg/ml for phosphoric acid-swollen cellulose, 1.3 mg/ml for carboxymethyl cellulose, 4.8 mg/ml for hydroxyethyl cellulose, 2.1 mg/ml for laminarin, 0.5 mg/ml for beta-glucan, 0.3 mg/ml for xyloglucan Trichoderma reesei
3.2.1.151 0.12
-
 XXXG 37°C, pH 5 Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.151 Trichoderma reesei
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.151 beta-glucan + H2O
-
 Trichoderma reesei beta-glucan oligosaccharides
-
 ?
3.2.1.151 carboxymethyl cellulose + H2O
-
 Trichoderma reesei carboxymethyl cellulose oligosaccharides
-
 ?
3.2.1.151 Glcbeta(1-3)Glcbeta(1-3)Glc + H2O
-
 Trichoderma reesei D-glucose + Glcbeta(1-3)Glc 38% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc ?
3.2.1.151 Glcbeta(1-4)Glc(1-4)Glc + H2O
-
 Trichoderma reesei D-glucose + Glcbeta(1-4)Glc 32% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc ?
3.2.1.151 Glcbeta(1-4)Glc(1-4)Glcbeta(1-4)Glc + H2O
-
 Trichoderma reesei Glcbeta(1-4)Glc 76% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc ?
3.2.1.151 Glcbeta(1-4)Glc-2-chloro-4-nitrophenol + H2O 24% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc Trichoderma reesei D-glucose + 2-chloro-4-nitrophenyl-beta-D-glucoside
-
 ?
3.2.1.151 Glcbeta(1-4)Glcbeta(1-3)Glc + H2O
-
 Trichoderma reesei D-glucose + Glcbeta(1-3)Glc 52% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc ?
3.2.1.151 Glcbeta(1-4)Glcbeta(1-4)Glc(1-4)Glc-2-chloro-4-nitrophenol + H2O
-
 Trichoderma reesei Glcbeta(1-4)Glc(1-4)Glc + 2-chloro-4-nitrophenyl-beta-D-glucoside 84% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc ?
3.2.1.151 Glcbeta(1-4)Glcbeta(1-4)Glc-2-chloro-4-nitrophenol + H2O
-
 Trichoderma reesei Glcbeta(1-4)Glc + 2-chloro-4-nitrophenyl-beta-D-glucoside 68% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc ?
3.2.1.151 Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-3)Glc + H2O
-
 Trichoderma reesei Glcbeta(1-4)Glc + Glcbeta(1-3)Glc 83% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc ?
3.2.1.151 Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc + H2O
-
 Trichoderma reesei Glcbeta(1-4)Glcbeta(1-4)Glc + GG
-
 ?
3.2.1.151 hydroxyethyl cellulose + H2O
-
 Trichoderma reesei hydroxyethyl cellulose oligosaccharides
-
 ?
3.2.1.151 laminarin + H2O
-
 Trichoderma reesei laminarin oligosaccharides
-
 ?
3.2.1.151 phosphoric acid-swollen cellulose + H2O
-
 Trichoderma reesei ?
-
 ?
3.2.1.151 XXXG + H2O X stands for a substituted glucose residue, G for an unsubstituted glucose residue Trichoderma reesei XX + XG 270% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc ?
3.2.1.151 XXXGXXXG + H2O X stands for a substituted glucose residue, G for an unsubstituted glucose residue Trichoderma reesei XX + XGXX + XG hydrolysis at both chain ends of substrate. 390% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc ?
3.2.1.151 xyloglucan + H2O hydrolysis at substituted glucose residues Trichoderma reesei xyloglucan oligosaccharides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.151 Cel74A
-
 Trichoderma reesei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.151 60
-
-
 Trichoderma reesei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.151 7
-
 phosphoric acid-swollen cellulose 37°C, pH 5 Trichoderma reesei
3.2.1.151 7.2
-
 XXXG 37°C, pH 5 Trichoderma reesei
3.2.1.151 52
-
 carboxymethyl cellulose 37°C, pH 5 Trichoderma reesei
3.2.1.151 138
-
 hydroxyethyl cellulose 37°C, pH 5 Trichoderma reesei
3.2.1.151 193
-
 Laminarin 37°C, pH 5 Trichoderma reesei
3.2.1.151 306
-
 beta-Glucan 37°C, pH 5 Trichoderma reesei
3.2.1.151 805
-
 xyloglucan 37°C, pH 5 Trichoderma reesei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.151 5
-
-
 Trichoderma reesei