Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tang, K.; Utairungsee, T.; Kanokratana, P.; Sriprang, R.; Champreda, V.; Eurwilaichitr, L.; Tanapongpipat, S.
    Characterization of a novel cyclomaltodextrinase expressed from environmental DNA isolated from Bor Khleung hot spring in Thailand (2006), FEMS Microbiol. Lett., 260, 91-99.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.54 expression in Escherichia coli and Pichia pastoris unidentified

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.54 4.4
-
 alpha-cyclodextrin
-
 unidentified
3.2.1.54 6.1
-
 beta-cyclodextrin
-
 unidentified

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.54 Ca2+ required for full activity unidentified

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.54 68000
-
 SDS-PAGE, size exclusion chromatography unidentified

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 unidentified
-
 Env Cda13A can be classified into the neopullulanase subfamily consisting of neopullulanases, cyclomaltodextrinases and maltogenic amylases
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54 of the recombinant protein by filtration of the supernatant unidentified

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.54 0.1
-
 starch as substrate unidentified
3.2.1.54 0.3
-
 pullulan as substrate unidentified
3.2.1.54 3.4
-
 gamma-cyclodextrin as substrate unidentified
3.2.1.54 14.8
-
 beta-cyclodextrin as substrate unidentified
3.2.1.54 30.7
-
 alpha-cyclodextrin as substrate unidentified

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 alpha-cyclodextrin + H2O
-
 unidentified maltose + D-glucose
-
 ?
3.2.1.54 beta-cyclodextrin + H2O
-
 unidentified maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 cyclomaltodextrin + H2O
-
 unidentified linear maltodextrin
-
 ?
3.2.1.54 gamma-cyclodextrin + H2O
-
 unidentified maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 maltooligosaccharides + H2O
-
 unidentified D-glucose + ?
-
 ?
3.2.1.54 pullulan + H2O
-
 unidentified maltotriose + ?
-
 ?
3.2.1.54 starch + H2O
-
 unidentified D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.54 monomer
-
 unidentified

Synonyms

EC Number Synonyms Comment Organism
3.2.1.54 CDase
-
 unidentified
3.2.1.54 cyclomaltodextrinase
-
 unidentified
3.2.1.54 Env cda13A
-
 unidentified

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.54 50 55
-
 unidentified

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.54 6 7
-
 unidentified