Literature summary extracted from
Rodrigo, D.; Cortes, C.; Clynen, E.; Schoofs, L.; Van Loey, A.; Hendrickx, M.
Thermal and high-pressure stability of purified polygalacturonase and pectinmethylesterase from four different tomato processing varieties (2006), Food Res. Int., 39, 440-448.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.2.1.15 |
food industry |
high pressure processing can be used for selective inactivation of PG in tomato processing while keeping pectinmethylesterase intact |
Solanum lycopersicum |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.2.1.15 |
additional information |
PG1 and PG2 can be inactivated at room temperature in the pressure range of 300-500 MPa |
Solanum lycopersicum |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.2.1.15 |
43000 |
- |
43000, SDS-PAGE, 43700, Maldi-Tof-spec analysis, PG1 and PG2 |
Solanum lycopersicum |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.15 |
Solanum lycopersicum |
- |
varieties Galeon, Malpica, Perfectpeel and Soto |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.15 |
by cation exchange chromatography |
Solanum lycopersicum |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
3.2.1.15 |
fruit |
ripe tomato. Varieties Galeon and Soto have about 50% of each enzyme, whereas Malpica and Perfectpeel contain more PG1 than PG2 |
Solanum lycopersicum |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.2.1.15 |
polygalacturonic acid + H2O |
- |
Solanum lycopersicum |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.2.1.15 |
? |
43000, SDS-PAGE, 43700, Maldi-Tof-spec analysis, PG1 and PG2 |
Solanum lycopersicum |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.2.1.15 |
PG1 |
- |
Solanum lycopersicum |
3.2.1.15 |
PG2 |
- |
Solanum lycopersicum |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.2.1.15 |
additional information |
- |
inactivation at around 55°C corresponds to PG2 and at around 80°C corresponds to PG1. After 5 min at 90°C almost complete inactivation |
Solanum lycopersicum |