Literature summary extracted from
Fusi, P.; Grisa, M.; Mombelli, E.; Consonni, R.; Tortora, P.; Vanoni, M.
Expression of a synthetic gene encoding P2 ribonuclease from the extreme thermoacidophilic archaebacterium Sulfolobus solfataricus in mesophylic hosts. (1995), Gene, 154, 99-103.
Application
EC Number |
Application |
Comment |
Organism |
---|
4.6.1.18 |
synthesis |
expression of enzyme in Escherichia coli and Saccharomyces cerevisiae. Recombinant proteins are indistinguishable from the Sulfolobus solfataricus enzyme on the basis of heat stability, pH optimum and RNA digestion pattern as well as NMR analysis, the only exceptions being that residues K4 and K6 are not methylated in the recombinant enzyme |
Saccharolobus solfataricus |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.6.1.18 |
expression in Escherichia coli and Saccharomyces cerevisiae. Recombinant proteins are indistinguishable from the Sulfolobus solfataricus enzyme on the basis of heat stability, pH optimum and RNA digestion pattern as well as NMR analysis, the only exceptions being that residues K4 and K6 are not methylated in the recombinant enzyme |
Saccharolobus solfataricus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.6.1.18 |
Saccharolobus solfataricus |
- |
isoform P2 |
- |