Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fusi, P.; Grisa, M.; Mombelli, E.; Consonni, R.; Tortora, P.; Vanoni, M.
    Expression of a synthetic gene encoding P2 ribonuclease from the extreme thermoacidophilic archaebacterium Sulfolobus solfataricus in mesophylic hosts. (1995), Gene, 154, 99-103.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.6.1.18 synthesis expression of enzyme in Escherichia coli and Saccharomyces cerevisiae. Recombinant proteins are indistinguishable from the Sulfolobus solfataricus enzyme on the basis of heat stability, pH optimum and RNA digestion pattern as well as NMR analysis, the only exceptions being that residues K4 and K6 are not methylated in the recombinant enzyme Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.18 expression in Escherichia coli and Saccharomyces cerevisiae. Recombinant proteins are indistinguishable from the Sulfolobus solfataricus enzyme on the basis of heat stability, pH optimum and RNA digestion pattern as well as NMR analysis, the only exceptions being that residues K4 and K6 are not methylated in the recombinant enzyme Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.18 Saccharolobus solfataricus
-
isoform P2
-