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Literature summary extracted from
- Mutations on aromatic residues of the active site to alter selectivity of the Sulfolobus solfataricus maltooligosyltrehalose synthase (2006), J. Agric. Food Chem., 54, 3585-3590.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.15 | expression in Escherichia coli | Saccharolobus solfataricus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.15 | F405Y | mutation located near subsite +1 constructed to alter enzyme selectivity. Catalytic efficiency for transglycosylation reaction is similar to wild-type, for hydrolysis somewhat lower than wild-type | Saccharolobus solfataricus |
| 5.4.99.15 | Y290F | mutation located near subsite +1 constructed to alter enzyme selectivity. Catalytic efficiency for hydrolysis and transglycosylation reaction are 6.6 and 5.6% resp., of wild-type | Saccharolobus solfataricus |
| 5.4.99.15 | Y367F | mutation located near subsite +1 constructed to alter enzyme selectivity. Catalytic efficiency for hydrolysis and transglycosylation reaction are about half of wild-type | Saccharolobus solfataricus |
| 5.4.99.15 | Y409F | mutation located near subsite +1 constructed to alter enzyme selectivity. Catalytic efficiency for hydrolysis is similar to wild-type, for transglycosylation reaction somewhat lower than wild-type | Saccharolobus solfataricus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.15 | 3.78 | - |
maltohexaose | mutant Y367F, pH 5.0, 60°C | Saccharolobus solfataricus |  |
| 5.4.99.15 | 3.8 | - |
maltoheptaose | mutant F405Y, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 3.8 | - |
maltoheptaose | mutant Y409F, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 3.88 | - |
maltoheptaose | wild-type, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 4.53 | - |
maltohexaose | wild-type, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 4.9 | - |
maltohexaose | mutant F405Y, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 5.85 | - |
maltopentaose | mutant Y409F, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 5.94 | - |
maltopentaose | wild-type, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 6.26 | - |
maltohexaose | mutant Y409F, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 6.34 | - |
maltopentaose | mutant F405Y, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 11.6 | - |
maltotetraose | mutant F405Y, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 13.2 | - |
maltotetraose | wild-type, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 16.1 | - |
maltotetraose | mutant Y409F, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 26.5 | - |
maltohexaose | mutant Y290F, pH 5.0, 60°C | Saccharolobus solfataricus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.15 | Saccharolobus solfataricus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.15 | recombinant wild-type and mutants | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.15 | maltoheptaose | - |
Saccharolobus solfataricus | alpha-maltopentaosyl trehalose | - |
? | |
| 5.4.99.15 | maltohexaose | - |
Saccharolobus solfataricus | alpha-maltotetraosyl trehalose | - |
? | |
| 5.4.99.15 | Maltopentaose | - |
Saccharolobus solfataricus | alpha-Maltotriosyl trehalose | - |
? | |
| 5.4.99.15 | Maltotetraose | - |
Saccharolobus solfataricus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.15 | maltooligosyltrehalose synthase | - |
Saccharolobus solfataricus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.15 | 26.5 | - |
maltohexaose | mutant Y290F, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 114 | - |
maltotetraose | mutant Y409F, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 138 | - |
maltotetraose | wild-type, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 139 | - |
maltotetraose | mutant F405Y, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 139 | - |
maltohexaose | mutant Y367F, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 267 | - |
maltopentaose | mutant Y409F, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 282 | - |
maltoheptaose | mutant Y409F, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 330 | - |
maltohexaose | mutant Y409F, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 354 | - |
maltopentaose | mutant F405Y, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 359 | - |
maltohexaose | wild-type, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 362 | - |
maltohexaose | mutant F405Y, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 363 | - |
maltoheptaose | mutant F405Y, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 364 | - |
maltopentaose | wild-type, pH 5.0, 60°C | Saccharolobus solfataricus | |
| 5.4.99.15 | 383 | - |
maltoheptaose | wild-type, pH 5.0, 60°C | Saccharolobus solfataricus | |
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