EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.141 | additional information | EDTA does not activate. Expression levels of both wild-type and His-tagged MTHases are higher in the absence of IPTG than in the presence of 0.5 mM IPTG induction | Saccharolobus solfataricus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.141 | pET-15b-deltaH-treZ vector expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL | Saccharolobus solfataricus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.141 | Cu2+ | inhibits at 4 mM | Saccharolobus solfataricus | |
3.2.1.141 | Hg2+ | 0.4 and 4 mM strongly inhibit | Saccharolobus solfataricus | |
3.2.1.141 | additional information | EDTA, Na+, K+, Mg2+, Ca2+, and Zn2+ do not inhibit | Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.141 | 2.63 | - |
maltoheptaose | - |
Saccharolobus solfataricus | |
3.2.1.141 | 5.63 | - |
maltohexaose | - |
Saccharolobus solfataricus | |
3.2.1.141 | 5.66 | - |
maltotetraosyl trehalose | - |
Saccharolobus solfataricus | |
3.2.1.141 | 5.86 | - |
maltopentaose | - |
Saccharolobus solfataricus | |
3.2.1.141 | 5.89 | - |
maltopentaosyl trehalose | - |
Saccharolobus solfataricus | |
3.2.1.141 | 7.22 | - |
maltotriosyl trehalose | - |
Saccharolobus solfataricus | |
3.2.1.141 | 10.9 | - |
maltotetraose | - |
Saccharolobus solfataricus | |
3.2.1.141 | 11.1 | - |
maltosyl trehalose | - |
Saccharolobus solfataricus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.141 | additional information | Na+, K+, Mg2+, Ca2+, and Zn2+ do not activate | Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.141 | 64000 | - |
2 * 64000, SDS-PAGE, 2 * 64240, electrospray ionization mass spectrometry, wild-type enzyme | Saccharolobus solfataricus |
3.2.1.141 | 64240 | - |
2 * 64000, SDS-PAGE, 2 * 64240, electrospray ionization mass spectrometry, wild-type enzyme | Saccharolobus solfataricus |
3.2.1.141 | 66000 | - |
1 * 66000, SDS-PAGE, His-tagged enzyme | Saccharolobus solfataricus |
3.2.1.141 | 68700 | - |
gel filtration, His-tagged enzyme | Saccharolobus solfataricus |
3.2.1.141 | 128800 | - |
gel filtration, wild-type enzyme | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.141 | Saccharolobus solfataricus | P95867 | ATCC 35092 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.141 | wild-type enzyme purified by heat treatment, nucleic acid precipitation, and ion-exchange chromatography, 12fold. His-tagged MTHase partially purified from the cell-free extract directly by metal chelating chromatography, 14.7fold | Saccharolobus solfataricus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.141 | 8 | 12 | wild-type, 12fold purified | Saccharolobus solfataricus |
3.2.1.141 | 21.1 | - |
His-tagged enzyme, crude extract | Saccharolobus solfataricus |
3.2.1.141 | 67.5 | - |
wild-type, crude extract | Saccharolobus solfataricus |
3.2.1.141 | 310 | - |
His-tagged enzyme, 14.7fold purified | Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.141 | maltoheptaose + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
3.2.1.141 | maltohexaose + H2O | for glucose formation, maltohexaose exhibits the highest formation rate | Saccharolobus solfataricus | ? | - |
? | |
3.2.1.141 | maltopentaose + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
3.2.1.141 | maltopentaosyltrehalose + H2O | trehalose formation rate increases as the degree of polymerization value of the substrate increases. maltopentaosyl trehalose is the most preferred substrate for trehalose formation | Saccharolobus solfataricus | maltopentaose + alpha,alpha'-trehalose | - |
? | |
3.2.1.141 | maltosyltrehalose + H2O | - |
Saccharolobus solfataricus | maltose + alpha,alpha'-trehalose | - |
? | |
3.2.1.141 | maltotetraose + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
3.2.1.141 | maltotetraosyltrehalose + H2O | - |
Saccharolobus solfataricus | maltotetraose + alpha,alpha'-trehalose | - |
? | |
3.2.1.141 | maltotriosyltrehalose + H2O | - |
Saccharolobus solfataricus | maltotriose + alpha,alpha'-trehalose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.141 | homodimer | 2 * 64000, SDS-PAGE, 2 * 64240, electrospray ionization mass spectrometry, wild-type enzyme | Saccharolobus solfataricus |
3.2.1.141 | monomer | 1 * 66000, SDS-PAGE, His-tagged enzyme | Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.141 | Maltooligosyl trehalose trehalohydrolase | - |
Saccharolobus solfataricus |
3.2.1.141 | MTHase | - |
Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.141 | 85 | - |
- |
Saccharolobus solfataricus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.141 | 45 | 85 | - |
Saccharolobus solfataricus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.141 | 85 | - |
retains all of its activity for 2 h | Saccharolobus solfataricus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.141 | additional information | - |
maltoheptaose | - |
Saccharolobus solfataricus | |
3.2.1.141 | 5.49 | - |
maltotetraose | - |
Saccharolobus solfataricus | |
3.2.1.141 | 6.01 | - |
maltoheptaose | - |
Saccharolobus solfataricus | |
3.2.1.141 | 17.7 | - |
maltopentaose | - |
Saccharolobus solfataricus | |
3.2.1.141 | 18.2 | - |
maltohexaose | - |
Saccharolobus solfataricus | |
3.2.1.141 | 193 | - |
maltosyl trehalose | - |
Saccharolobus solfataricus | |
3.2.1.141 | 1030 | - |
maltotriosyl trehalose | - |
Saccharolobus solfataricus | |
3.2.1.141 | 1190 | - |
maltotetraosyl trehalose | - |
Saccharolobus solfataricus | |
3.2.1.141 | 1230 | - |
maltopentaosyl trehalose | - |
Saccharolobus solfataricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.141 | 5 | - |
- |
Saccharolobus solfataricus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.141 | 3.5 | 11 | - |
Saccharolobus solfataricus |