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Literature summary extracted from

  • Rolfsmeier, M.; Haseltine, C.; Bini, E.; Clark, A.; Blum, P.
    Molecular characterization of the alpha-glucosidase gene (malA) from the hyperthermophilic archaeon Sulfolobus solfataricus (1998), J. Bacteriol., 180, 1287-1295.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.20 gene malA, library preparation, DNA and amino acid sequence determination and analysis, genetic structure and organization, sequences 3' to position -33, including a consensus archaeal TATA box, play an essential role in malA expression, detection of malA homologues, overview, phylogenetic analysis, expression of MalA in Escherichia coli revealing differences from the native enzyme in thermostability and electrophoretic behavior, overview Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 64.9
-
 glycogen pH 5.5, 85°C, recombinant enzyme Saccharolobus solfataricus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.20 soluble
-
 Saccharolobus solfataricus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 80500
-
 x * 80500, about, sequence calculation Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.20 maltose + H2O Saccharolobus solfataricus
-
 alpha-D-glucose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Saccharolobus solfataricus P0CD66 malA; strains 98/2 and P2, gene malA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 glycogen + H2O recombinant enzyme Saccharolobus solfataricus alpha-D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Saccharolobus solfataricus alpha-D-glucose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 ? x * 80500, about, sequence calculation Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 MalA
-
 Saccharolobus solfataricus
3.2.1.20 maltase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 85
-
 recombinant enzyme, substrate glycogen Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 5.5
-
 recombinant enzyme, substrate glycogen Saccharolobus solfataricus