EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.22 | expressed in Escherichia coli strain BL21(DE3)-RIL | Saccharolobus solfataricus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.22 | Ag2+ | complete inhibition at 10 mM | Saccharolobus solfataricus | |
3.2.1.22 | Ca2+ | complete inhibition at 10 mM | Saccharolobus solfataricus | |
3.2.1.22 | Cd2+ | complete inhibition at 10 mM | Saccharolobus solfataricus | |
3.2.1.22 | Co2+ | complete inhibition at 10 mM | Saccharolobus solfataricus | |
3.2.1.22 | Cu2+ | complete inhibition at 10 mM | Saccharolobus solfataricus | |
3.2.1.22 | Hg2+ | complete inhibition at 10 mM | Saccharolobus solfataricus | |
3.2.1.22 | Mn2+ | complete inhibition at 10 mM | Saccharolobus solfataricus | |
3.2.1.22 | additional information | D-galactose, L-arabinose, D-fucose, sucrose, EDTA, dithiothreitol, Mg2+ and Mo2+ have no inhibitory effect | Saccharolobus solfataricus | |
3.2.1.22 | Ni2+ | complete inhibition at 10 mM | Saccharolobus solfataricus | |
3.2.1.22 | Zn2+ | complete inhibition at 10 mM | Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.22 | 0.085 | - |
p-nitrophenyl alpha-D-galactopyranoside | in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80°C | Saccharolobus solfataricus | |
3.2.1.22 | 37.4 | - |
p-nitrophenyl-beta-L-arabinopyranoside | in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80°C | Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 225000 | - |
gel filtration | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.22 | Saccharolobus solfataricus | Q97U94 | - |
- |
3.2.1.22 | Saccharolobus solfataricus P2 | Q97U94 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.22 | Q-Sepharose Fast Flow column chromatography, hydroxyapatite column chromatography, Mono Q 5/50 GL column chromatography, and Superdex 200 HR 10/30 gel filtration | Saccharolobus solfataricus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 0.18 | - |
crude cell extract, in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80°C | Saccharolobus solfataricus |
3.2.1.22 | 4.25 | - |
after 23fold purification, in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80°C | Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.22 | melibiose + H2O | - |
Saccharolobus solfataricus | D-galactose + D-glucose | - |
? | |
3.2.1.22 | melibiose + H2O | - |
Saccharolobus solfataricus P2 | D-galactose + D-glucose | - |
? | |
3.2.1.22 | additional information | shows no activity toward p-nitrophenyl-alpha-substituted hexoses such as D-glucose, D-mannose, L-rhamnose, or N-acetyl-D-galactosaminide, p-nitrophenyl-beta-substituted hexoses such as D-galactose, D-glucose, and D-mannose do not support catalysis, nor does the pentose p-nitrophenyl-beta-D-xylopyranoside | Saccharolobus solfataricus | ? | - |
? | |
3.2.1.22 | additional information | shows no activity toward p-nitrophenyl-alpha-substituted hexoses such as D-glucose, D-mannose, L-rhamnose, or N-acetyl-D-galactosaminide, p-nitrophenyl-beta-substituted hexoses such as D-galactose, D-glucose, and D-mannose do not support catalysis, nor does the pentose p-nitrophenyl-beta-D-xylopyranoside | Saccharolobus solfataricus P2 | ? | - |
? | |
3.2.1.22 | p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | p-nitrophenol + alpha-D-galactose | - |
? | |
3.2.1.22 | p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus P2 | p-nitrophenol + alpha-D-galactose | - |
? | |
3.2.1.22 | p-nitrophenyl-beta-L-arabinopyranoside + H2O | - |
Saccharolobus solfataricus | p-nitrophenol + beta-L-arabinose | - |
? | |
3.2.1.22 | raffinose + H2O | - |
Saccharolobus solfataricus | sucrose + alpha-D-galactose | - |
? | |
3.2.1.22 | raffinose + H2O | - |
Saccharolobus solfataricus P2 | sucrose + alpha-D-galactose | - |
? | |
3.2.1.22 | stachyose + 2 H2O | - |
Saccharolobus solfataricus | 2 alpha-D-galactose + sucrose | - |
? | |
3.2.1.22 | stachyose + 2 H2O | - |
Saccharolobus solfataricus P2 | 2 alpha-D-galactose + sucrose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.22 | trimer | - |
Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.22 | alpha-Gal | - |
Saccharolobus solfataricus |
3.2.1.22 | GalS | - |
Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 90 | - |
- |
Saccharolobus solfataricus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 50 | - |
completely inactive at temperatures below 50°C | Saccharolobus solfataricus |
3.2.1.22 | 75 | - |
50% active at 75°C | Saccharolobus solfataricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 5 | - |
- |
Saccharolobus solfataricus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 4.1 | 6.7 | more than 50% active between pH 4.1 and 6.7 | Saccharolobus solfataricus |