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Literature summary extracted from

  • Banerjee, S.; Nandyala, A.K.; Raviprasad, P.; Ahmed, N.; Hasnain, S.E.
    Iron-dependent RNA-binding activity of Mycobacterium tuberculosis aconitase (2007), J. Bacteriol., 189, 4046-4052.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.3 0.56
-
 isocitrate pH 8.0, 25°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.3 Iron iron is required for aconitase activity, but inhibits the RNA-binding activity, the two activities are mutually exclusive Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.3 100000
-
 gel filtration Mycobacterium tuberculosis
4.2.1.3 102000
-
 1 * 102000, SDS-PAGE Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.3 Mycobacterium tuberculosis
-
 bifunctional enzyme with aconitase activity and working as iron-responsive protein. Since iron is required for aconitase activity, but inhibits the RNA-binding activity, the two activities are mutually exclusive
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.3 118
-
 pH 8.0, 25°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.3 isocitrate
-
 Mycobacterium tuberculosis cis-aconitate + H2O
-
 ?
4.2.1.3 additional information in addition to aconitase activity, enzyme binds with high specificity to iron-responsive element-like RNA sequences. Iron is required for aconitase activity, but inhibits the RNA-binding activity, the two activities are mutually exclusive Mycobacterium tuberculosis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.3 monomer 1 * 102000, SDS-PAGE Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.3 8
-
-
 Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.3 7 10
-
 Mycobacterium tuberculosis