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Literature summary extracted from

  • del Castillo, T.; Ramos, J.L.; Rodriguez-Herva, J.J.; Fuhrer, T.; Sauer, U.; Duque, E.
    Convergent peripheral pathways catalyze initial glucose catabolism in Pseudomonas putida: genomic and flux analysis (2007), J. Bacteriol., 189, 5142-5152.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.2 additional information the enzyme is 2.12fold induced by growth on glucose compared to citrate Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.2 gene glk is organized in gene clusters, transcriptomic analysis of KT2440 in response to glucose, overview Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.2 additional information site-specific homologous inactivation of gene glk to construct a mutant strain, activity of the enzyme in isogenic mutants with mutations in the glucose metabolism network, glk mutants exhibit a lower total carbon consumption rate than the parental strain, overview Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.2 cytoplasm
-
 Pseudomonas putida 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.2 ATP + D-glucose Pseudomonas putida the enzyme initiates the intracellular glucose catabolism/metabolism, overview ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ATP + D-glucose Pseudomonas putida KT 2240 the enzyme initiates the intracellular glucose catabolism/metabolism, overview ADP + D-glucose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.2 Pseudomonas putida
-
 and isogenic mutant strains, gene glk
-
2.7.1.2 Pseudomonas putida KT 2240
-
 and isogenic mutant strains, gene glk
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.2 additional information
-
 activity of the enzyme in isogenic mutants with mutations in the glucose metabolism network, overview Pseudomonas putida
2.7.1.2 0.0018
-
 strain KT2440, grown on citrate Pseudomonas putida
2.7.1.2 0.0211
-
 strain KT2440, grown on glucose Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.2 ATP + D-glucose
-
 Pseudomonas putida ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ATP + D-glucose the enzyme initiates the intracellular glucose catabolism/metabolism, overview Pseudomonas putida ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ATP + D-glucose
-
 Pseudomonas putida KT 2240 ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ATP + D-glucose the enzyme initiates the intracellular glucose catabolism/metabolism, overview Pseudomonas putida KT 2240 ADP + D-glucose 6-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.2 glk
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.2 30
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.2 7
-
 assay at Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.2 ATP
-
 Pseudomonas putida