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Literature summary extracted from

  • Yamano, S.; Maruyama, T.
    An azide-insensitive superoxide dismutase from a hyperthermophilic archaeon, Sulfolobus solfataricus (1999), J. Biochem., 125, 186-193.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 DNA and amino acid sequence determination and anaylsis, expression of wild-type and mutant in Escherichia coli Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
1.15.1.1 Y88F site-directed mutagenesis, substitution of Tyr88 to Phe does not affect the metal specificity of the enzyme Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 fluoride recombinant Fe-reconstituted SOD Saccharolobus solfataricus
1.15.1.1 H2O2 distinguishes Fe-SOD from Mn-SOD, since it inactivates only Fe-SOD Saccharolobus solfataricus
1.15.1.1 additional information recombinant Fe-reconstituted SOD is not inhibited by azide, steric hindrance in the substrate funnel of the enzyme prevents the access of N3- but allows O2- and F- access to the active site Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Fe2+ Fe/Mn-type SOD Saccharolobus solfataricus
1.15.1.1 Mn2+ Fe/Mn-type SOD Saccharolobus solfataricus
1.15.1.1 additional information metal content analysis of the recombinant enzyme Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Saccharolobus solfataricus P80857 DSM 1616
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 recombinant wild-type and mutant from Escherichia coli Saccharolobus solfataricus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.15.1.1 reconstitution of Fe-SOD from purified recombinant apo-enzyme, apo-enzyme preparation: purified recombinant enzyme is denatured in buffer containing 50 mM acetate buffer, pH 3.8, 6 M guanidine hydrochloride, and 10 mM EDTA for 16 h at 50°C, followed by dialysis against the same buffer containing MnSO4 instead of EDTA, and removal of guanidine hydrochloride in a second dialysis step, followed by gel filtration, overview Saccharolobus solfataricus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.15.1.1 additional information Sulfolobus solfataricus grows between 50°C and 87°C with an optimum growing temperature of 87°C, but it neither grows nor survives at 90°C Saccharolobus solfataricus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 1.2
-
 reconstituted Mn-SOD Saccharolobus solfataricus
1.15.1.1 4.3
-
 reconstituted Mn-SOD mutant Y88F Saccharolobus solfataricus
1.15.1.1 481
-
 reconstituted Fe-SOD Saccharolobus solfataricus
1.15.1.1 755
-
 reconstituted Fe-SOD mutant Y88F Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 SOD
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.1 25
-
 assay at Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 95
-
 2 h, purified recombinant enzyme, 96% remaining activity, half-life is 33 h, inactivation according to first order kinetics Saccharolobus solfataricus
1.15.1.1 100
-
 purified recombinant enzyme, half-life is 8.7 h, inactivation according to first order kinetics Saccharolobus solfataricus
1.15.1.1 105
-
 purified recombinant enzyme, half-life is 1.5 h, inactivation according to first order kinetics Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 7.8
-
 assay at Saccharolobus solfataricus