Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ikeda, C.; Hayashi, Y.; Itoh, N.; Seto, H.; Dairi, T.
    Functional analysis of eubacterial ent-copalyl diphosphate synthase and pimara-9(11),15-diene synthase with unique primary sequences (2007), J. Biochem., 141, 37-45.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.31 2-mercaptoethanol 5 mM, required for full activity Streptomyces sp.
4.2.3.31 Tween-80 0.1%, required for full activity Streptomyces sp.
5.5.1.13 2-mercaptoethanol 5 mM, required for full activity Streptomyces sp.
5.5.1.13 Tween-80 0.1%, required for full activity Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.5.1.13 geranylgeranyl diphosphate inhibitory above 0.03 mM Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.31 0.00026
-
 ent-copalyl diphosphate 30°C, pH 7.0 Streptomyces sp.
5.5.1.13 0.0137
-
 geranylgeranyl diphosphate 35°C, pH 5.5 Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.31 Co2+ 1 mM, 100% of the activity with Mg2+ Streptomyces sp.
4.2.3.31 Mg2+ absolutely required, optimal concentraion 1 mM Streptomyces sp.
4.2.3.31 additional information no or very weak activity in presence of Mn2+, Ca2+, Cu2+ and Fe2+ Streptomyces sp.
4.2.3.31 Ni2+ 1 mM, 57% of the activity with Mg2+ Streptomyces sp.
4.2.3.31 Zn2+ 1 mM, 59% of the activity with Mg2+ Streptomyces sp.
5.5.1.13 Co2+ or Zn2+, Mg2+, required. Activation in decreasing order: Mg2+, Zn2+, Co2+ Streptomyces sp.
5.5.1.13 Mg2+ or Zn2+, Co2+, required. Activation in decreasing order: Mg2+, Zn2+, Co2+ Streptomyces sp.
5.5.1.13 Zn2+ or Mg2+, Co2+, required. Activation in decreasing order: Mg2+, Zn2+, Co2+ Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.31 35000
-
 gel filtration Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.31 Streptomyces sp.
-
-
-
4.2.3.31 Streptomyces sp. KO-3988
-
-
-
5.5.1.13 Streptomyces sp.
-
-
-
5.5.1.13 Streptomyces sp. KO-3988
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.31 ent-copalyl diphosphate
-
 Streptomyces sp. pimara-9(11),15-diene + diphosphate
-
 ?
4.2.3.31 ent-copalyl diphosphate
-
 Streptomyces sp. KO-3988 pimara-9(11),15-diene + diphosphate
-
 ?
4.2.3.31 additional information no substrates: farnesyl diphosphate, geranylgeranyl diphosphate, terpentecin diphosphate Streptomyces sp. ?
-
 ?
4.2.3.31 additional information no substrates: farnesyl diphosphate, geranylgeranyl diphosphate, terpentecin diphosphate Streptomyces sp. KO-3988 ?
-
 ?
5.5.1.13 geranylgeranyl diphosphate
-
 Streptomyces sp. ent-copalyl diphosphate
-
 ?
5.5.1.13 geranylgeranyl diphosphate
-
 Streptomyces sp. KO-3988 ent-copalyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.31 monomer x * 35000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.31 30
-
 0.05 M Tris, pH 7.0 Streptomyces sp.
5.5.1.13 35
-
-
 Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.3.31 35
-
 pH 5.5, stable for at least 1 h Streptomyces sp.
5.5.1.13 35
-
 pH 5.5, stable for at least 1 h Streptomyces sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.31 0.0014
-
 ent-copalyl diphosphate 30°C, pH 7.0 Streptomyces sp.
5.5.1.13 0.033
-
 geranylgeranyl diphosphate 35°C, pH 5.5 Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.31 7
-
-
 Streptomyces sp.
5.5.1.13 5.5
-
-
 Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.31 6 8
-
 Streptomyces sp.
5.5.1.13 5 8 more than 90% of maximum activity within this range Streptomyces sp.