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Literature summary extracted from

  • Karadzic, I.; Masui, A.; Zivkovic, L.I.; Fujiwara, N.
    Purification and characterization of an alkaline lipase from Pseudomonas aeruginosa isolated from putrid mineral cutting oil as component of metalworking fluid (2006), J. Biosci. Bioeng., 102, 82-89.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 chloroform at 25% v/v, 30°C, 130% activity after 24 h, 140% after 48 h Pseudomonas aeruginosa
3.1.1.3 dimethylformamide at 25% v/v, 30°C, 120% activity after 24 h, 110% after 48 h Pseudomonas aeruginosa
3.1.1.3 DTT 20% activation at 5 mM Pseudomonas aeruginosa
3.1.1.3 EDTA 30% activation at 5 mM Pseudomonas aeruginosa
3.1.1.3 iodoacetic acid 10% activation at 5 mM Pseudomonas aeruginosa
3.1.1.3 sodium deoxycholate 300% activation at 5 mM Pseudomonas aeruginosa
3.1.1.3 Triton X-100 300% activation at 5 mM Pseudomonas aeruginosa
3.1.1.3 Tween 80 200% activation at 5 mM Pseudomonas aeruginosa

Application

EC Number Application Comment Organism
3.1.1.3 biotechnology the alkaline lipase is stable and active in organic solvents and a water-restricted environment and has a great potential as a biotechnological tool e.g. in organosynthetic reactions in water-restricted medium or in control and prevention of metalworking fluid putrification in the metal industry Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Ca2+ 50% inhibition at 5 mM Pseudomonas aeruginosa
3.1.1.3 Cu2+ 92% inhibition at 5 mM Pseudomonas aeruginosa
3.1.1.3 diisopropyl fluorophosphate 30% inhibition at 5 mM, 60% inhibition at 25 mM Pseudomonas aeruginosa
3.1.1.3 Hg2+ 94% inhibition at 5 mM Pseudomonas aeruginosa
3.1.1.3 Mg2+ 32% inhibition at 5 mM Pseudomonas aeruginosa
3.1.1.3 Mn2+ 47% inhibition at 5 mM Pseudomonas aeruginosa
3.1.1.3 additional information no inhibition by EDTA, DTT, iodoacetic acid, and o-phenanthroline Pseudomonas aeruginosa
3.1.1.3 PMSF 19% inhibition at 5 mM Pseudomonas aeruginosa
3.1.1.3 SDS 93% inhibition at 5 mM Pseudomonas aeruginosa
3.1.1.3 Zn2+ 85% inhibition at 5 mM Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular the enzyme is secreted to the culture medium Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 54000
-
 x * 54000, SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.3 triacylglycerol + H2O Pseudomonas aeruginosa
-
 diacylglycerol + a carboxylate
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 Acetone at 25% v/v, 30°C, 95% remaining activity after 24 h, 33% after 48 h Pseudomonas aeruginosa
3.1.1.3 Butanol at 25% v/v, 30°C, 10% remaining activity after 48 h Pseudomonas aeruginosa
3.1.1.3 chloroform at 25% v/v, 30°C, 130% activity after 24 h, 140% after 48 h Pseudomonas aeruginosa
3.1.1.3 dimethylformamide at 25% v/v, 30°C, 120% activity after 24 h, 110% after 48 h, 100% remaining activity after 30 min at 50°C Pseudomonas aeruginosa
3.1.1.3 Ethanol at 25% v/v, 30°C, 90% remaining activity after 24 h, 17% after 48 h, 55% remaining activity after 10 min at 50°C Pseudomonas aeruginosa
3.1.1.3 ethyl methyl ketone 75% remaining activity after 10 min at 50°C Pseudomonas aeruginosa
3.1.1.3 hexane at 25% v/v, 30°C, 100% remaining activity after 24 h, 64% after 48 h, 100% reaming activity after 15 min at 60°C, 50% reamining activity after 30 min at 60°C Pseudomonas aeruginosa
3.1.1.3 isopropanol at 25% v/v, 30°C, 65% remaining activity after 24 h, 16% after 48 h Pseudomonas aeruginosa
3.1.1.3 Methanol at 25% v/v, 30°C, 80% remaining activity after 24 h, 42% after 48 h, 100% remaining activity after 10 min at 50°C Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Pseudomonas aeruginosa
-
 strain san-ai, isolated from putrid mineral cutting oil, with pH 10.0, as component of metalworking fluid for cooling and lubrication in industrial metalworking processes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 native enzyme 12.5fold from culture broth by ammonium sulfate fractionation, hydrophobic interaction chromatography, dialysis, and gel filtration to homogeneity Pseudomonas aeruginosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 0.00006
-
 purified enzyme, 95% acetone, 30°C Pseudomonas aeruginosa
3.1.1.3 0.00012
-
 purified enzyme, 95% ethyl-methyl-ketone, 30°C Pseudomonas aeruginosa
3.1.1.3 0.00013
-
 purified enzyme, 95% n-hexane, 30°C Pseudomonas aeruginosa
3.1.1.3 0.0052
-
 purified enzyme, 95% ethanol, 30°C Pseudomonas aeruginosa
3.1.1.3 0.231
-
 purified enzyme, 95% methanol, 30°C Pseudomonas aeruginosa
3.1.1.3 473
-
 purified enzyme, buffer Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Pseudomonas aeruginosa 4-nitrophenol + palmitate
-
 ?
3.1.1.3 methyl oleate + H2O acylglycerol lipase activity, EC 3.1.1.23 Pseudomonas aeruginosa methanol + oleate
-
 ?
3.1.1.3 triacylglycerol + H2O
-
 Pseudomonas aeruginosa diacylglycerol + a carboxylate
-
 ?
3.1.1.3 triacylglycerol + H2O the enzyme acts on triacylglycerols with medium fatty acid chain length, substrate specificity, overview Pseudomonas aeruginosa diacylglycerol + a carboxylate
-
 ?
3.1.1.3 tributyrin + H2O
-
 Pseudomonas aeruginosa dibutyrin + butyrate
-
 ?
3.1.1.3 tricaprin + H2O high activity Pseudomonas aeruginosa dicaprin + caprate
-
 ?
3.1.1.3 tricaproin + H2O high activity Pseudomonas aeruginosa dicaproin + caproate
-
 ?
3.1.1.3 tricaprylin + H2O best substrate Pseudomonas aeruginosa dicaprylin + caprylate
-
 ?
3.1.1.3 triolein + H2O
-
 Pseudomonas aeruginosa diolein + oleate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 54000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 alkaline lipase
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 70
-
-
 Pseudomonas aeruginosa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 30 90
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 60
-
 15 min, purified native enzyme, 75% remaining activity Pseudomonas aeruginosa
3.1.1.3 70
-
 15 min, purified native enzyme, 30% remaining activity Pseudomonas aeruginosa
3.1.1.3 80
-
 complete inactivation within 10 min Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 11
-
-
 Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 4 11.5 activity range Pseudomonas aeruginosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 4 11.5 stable Pseudomonas aeruginosa