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Literature summary extracted from
- Isolation and characterization of a tomato non-specific lipid transfer protein involved in polygalacturonase-mediated pectin degradation (2007), J. Exp. Bot., 58, 1151-1160.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.15 | additional information | a 8 kDa protein (ACT) from the PG1 protein complex is capable of converting the gene product PG2 into a more active and heat-stable form, which increases PG-mediated pectin degradation in vitro and stimulates PG-mediated tissue breakdown in planta. Addition of the 8 kDa protein to tomato PG2 causes a 4fold to 5fold increase in activity | Solanum lycopersicum | |
| 3.2.1.15 | additional information | apple or peach lipid transfer proteins significantly increase PG activity (more than 2fold) and heat stability | Aspergillus niger |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.15 | 45000 | - |
1 * 45000, SDS-PAGE, PG2 | Solanum lycopersicum |
| 3.2.1.15 | 100000 | - |
gel filtration, PG1 | Solanum lycopersicum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.15 | Aspergillus niger | - |
- |
- |
| 3.2.1.15 | Solanum lycopersicum | - |
cultivar Counter | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.15 | - |
Aspergillus niger |
| 3.2.1.15 | by gel filtration | Solanum lycopersicum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.15 | fruit | high activity of PG2 in ripe tomato fruit pericarp tissue | Solanum lycopersicum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.15 | pectin + H2O | - |
Aspergillus niger | galacturonic acid oligomers | - |
? |  |
| 3.2.1.15 | pectin + H2O | - |
Solanum lycopersicum | galacturonic acid oligomers | - |
? | |
| 3.2.1.15 | polygalacturonic acid + H2O | - |
Aspergillus niger | ? | - |
? | |
| 3.2.1.15 | polygalacturonic acid + H2O | - |
Solanum lycopersicum | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.15 | ? | 1 * 45000, SDS-PAGE, PG2 | Solanum lycopersicum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.15 | PG1 | - |
Solanum lycopersicum |
| 3.2.1.15 | PG2 | - |
Solanum lycopersicum |
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Release 2023.1 (January 2023)
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