EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.106 | full-length Arabidopsis GCSI fused to GFP, stable expression in tobacco BY-2 cells | Arabidopsis thaliana |
3.2.1.113 | GFP-fusions are transferred into Agrobacterium tumefaciens by heat shock | Nicotiana tabacum |
3.2.1.113 | GFP-fusions are transferred into Agrobacterium tumefaciens by heat shock | Arabidopsis thaliana |
3.2.1.113 | GFP-fusions are transferred into Agrobacterium tumefaciens by heat shock | Glycine max |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.106 | additional information | removal of the first 11 amino acids of the GCSI cytoplasmic tail results in relocalization from the endoplasmic reticulum to the Golgi apparatus | Arabidopsis thaliana |
3.2.1.113 | additional information | ManI fusion constructs are derived from the full-length GFP fusion | Nicotiana tabacum |
3.2.1.113 | additional information | ManI fusion constructs are derived from the full-length GFP fusion | Arabidopsis thaliana |
3.2.1.113 | additional information | ManI fusion constructs are derived from the full-length GFP fusion | Solanum lycopersicum |
3.2.1.113 | additional information | ManI fusion constructs are derived from the full-length GFP fusion | Glycine max |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.113 | additional information | cycloheximide, protein synthesis inhibitor. After a 2 h treatment with the protein synthesis inhibitor cycloheximide, ER and Golgi labeling observed with ManI-GFP fusion remains unchanged, showing that the steady state localization of ManI-GFP is the Golgi and the ER | Nicotiana tabacum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.106 | endoplasmic reticulum | with cytoplasmic tail, which is crucial for endoplasmic reticulum retention, thus there is a targeting mechanism relying on protein-protein interaction | Arabidopsis thaliana | 5783 | - |
3.2.1.106 | membrane | endoplasmic reticulum resident membrane protein, the 18-amino acid long transmembrane domain of GCSI is not sufficient to target this glycosidase in the endoplasmic reticulum membrane | Arabidopsis thaliana | 16020 | - |
3.2.1.113 | endoplasmic reticulum | - |
Nicotiana tabacum | 5783 | - |
3.2.1.113 | endoplasmic reticulum | - |
Arabidopsis thaliana | 5783 | - |
3.2.1.113 | endoplasmic reticulum | - |
Solanum lycopersicum | 5783 | - |
3.2.1.113 | endoplasmic reticulum | - |
Glycine max | 5783 | - |
3.2.1.113 | Golgi apparatus | different fusion proteins analyzed to determine if the portion of ManI located in the Golgi lumen plays a role in the targeting of this glycosidase to the Golgi and the ER membranes | Nicotiana tabacum | 5794 | - |
3.2.1.113 | Golgi apparatus | different fusion proteins analyzed to determine if the portion of ManI located in the Golgi lumen plays a role in the targeting of this glycosidase to the Golgi and the ER membranes | Arabidopsis thaliana | 5794 | - |
3.2.1.113 | Golgi apparatus | different fusion proteins analyzed to determine if the portion of ManI located in the Golgi lumen plays a role in the targeting of this glycosidase to the Golgi and the ER membranes | Solanum lycopersicum | 5794 | - |
3.2.1.113 | Golgi apparatus | different fusion proteins analyzed to determine if the portion of ManI located in the Golgi lumen plays a role in the targeting of this glycosidase to the Golgi and the ER membranes | Glycine max | 5794 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.106 | Arabidopsis thaliana | - |
- |
- |
3.2.1.113 | Arabidopsis thaliana | - |
- |
- |
3.2.1.113 | Glycine max | Q9SEH8 | - |
- |
3.2.1.113 | Nicotiana tabacum | - |
- |
- |
3.2.1.113 | Solanum lycopersicum | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.113 | TBY-2 cell | - |
Nicotiana tabacum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.113 | alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + 4 H2O | - |
Nicotiana tabacum | alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + 4 D-mannose | - |
? | |
3.2.1.113 | alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + 4 H2O | - |
Arabidopsis thaliana | alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + 4 D-mannose | - |
? | |
3.2.1.113 | alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + 4 H2O | - |
Solanum lycopersicum | alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + 4 D-mannose | - |
? | |
3.2.1.113 | alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + 4 H2O | - |
Glycine max | alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + 4 D-mannose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.106 | GCSI | - |
Arabidopsis thaliana |
3.2.1.106 | glucosidase I | - |
Arabidopsis thaliana |
3.2.1.113 | alpha-1,2 mannosidase I | - |
Nicotiana tabacum |
3.2.1.113 | alpha-1,2 mannosidase I | - |
Arabidopsis thaliana |
3.2.1.113 | alpha-1,2 mannosidase I | - |
Solanum lycopersicum |
3.2.1.113 | alpha-1,2 mannosidase I | - |
Glycine max |
3.2.1.113 | ManI | - |
Nicotiana tabacum |
3.2.1.113 | ManI | - |
Arabidopsis thaliana |
3.2.1.113 | ManI | - |
Solanum lycopersicum |
3.2.1.113 | ManI | - |
Glycine max |