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Literature summary extracted from

  • Profotova, B.; Burketova, L.; Novotna, Z.; Martinec, J.; Valentova, O.
    Involvement of phospholipases C and D in early response to SAR and ISR inducers in Brassica napus plants (2006), Plant Physiol. Biochem., 44, 143-151.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.3 benzothiadiazole rapid activation Brassica napus
3.1.4.3 methyl jasmonate rapid activation Brassica napus
3.1.4.3 salicylic acid rapid activation Brassica napus
3.1.4.4 benzothiadiazole rapid activation of isoforms PLDalpha, PLDbeta/gamma, PLDdelta. Strongest response for PLDbeta/gamma Brassica napus
3.1.4.4 methyl jasmonate rapid activation of isoforms PLDalpha, PLDbeta/gamma, PLDdelta Brassica napus
3.1.4.4 salicylic acid rapid activation of isoforms PLDalpha, PLDbeta/gamma, PLDdelta Brassica napus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.3 cytosol
-
 Brassica napus 5829
-
3.1.4.3 microsome
-
 Brassica napus
-
-
3.1.4.4 cytosol
-
 Brassica napus 5829
-
3.1.4.4 microsome
-
 Brassica napus
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Brassica napus
-
-
-
3.1.4.4 Brassica napus
-
 phosphatidylinositol bisphosphate independent isoform PLDalpha, phosphatidylinositol 4,5-bisphosphate dependent isoform PLDbeta/gamma, oleate-stimulated PLDdelta
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.3 leaf
-
 Brassica napus
-
3.1.4.4 leaf
-
 Brassica napus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.3 0.002
-
 microsomal fraction Brassica napus
3.1.4.4 0.00031
-
 isoform PLDdelta, cytosolic fraction, presence of 0.1 mM Ca2+ and 0.6 mM oleate Brassica napus
3.1.4.4 0.0008
-
 isoform PLDdelta, microsomal fraction, presence of 0.1 mM Ca2+ and 0.6 mM oleate Brassica napus
3.1.4.4 0.1
-
 isoform PLDalpha, cytosolic fraction, presence of 120 mM Ca2+ and 10 mM sodium dodecylsulfate Brassica napus
3.1.4.4 0.15
-
 isoform PLDalpha, microsomal fraction, presence of 120 mM Ca2+ and 10 mM sodium dodecylsulfate Brassica napus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 N-palmitoyl-phosphatidylethanolamine + H2O
-
 Brassica napus phosphatidic acid + N-palmitoylethanolamine
-
 ?
3.1.4.4 phosphatidylcholine + H2O
-
 Brassica napus 1,2-diacylglycerophosphate + choline
-
 ?