Any feedback?
Literature summary extracted from
- The influence of sodium ion binding on factor IXa activity (2006), Thromb. Haemost., 95, 936-941.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.22 | vitamin K | dependent on | Homo sapiens |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.22 | antithrombin | inactivation, second-order-association rate constants in absence or presence of H5, a pentasaccharide fragment derived from heparin, in a Ca2+-dependent manner, but independent of Na+, overview | Homo sapiens | |
| 3.4.21.22 | EDTA | complete inhibition at 20 mM | Homo sapiens | |
| 3.4.21.22 | Rb+ | slightly inhibiting in presence of Ca2+ | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.22 | 1.7 | - |
CH3SO2-D-Leu-Gly-Arg-4-nitroanilide | pH 7.5, 22°C, in presence of 5 mM Ca2+ and 0.2 M ChCl | Homo sapiens | |
| 3.4.21.22 | 2.2 | - |
CH3SO2-D-Leu-Gly-Arg-4-nitroanilide | pH 7.5, 22°C, in presence of 5 mM Ca2+ with or without presence of 0.2 M LiCl | Homo sapiens | |
| 3.4.21.22 | 2.3 | - |
CH3SO2-D-Leu-Gly-Arg-4-nitroanilide | pH 7.5, 22°C, in presence of 5 mM Ca2+ and 0.2 M NaCl | Homo sapiens | |
| 3.4.21.22 | 3.2 | - |
CH3SO2-D-Leu-Gly-Arg-4-nitroanilide | pH 7.5, 22°C, in presence of 5 mM Ca2+ and 0.2 M RbCl | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.22 | extracellular | - |
Homo sapiens | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.22 | Ca2+ | activates | Homo sapiens | |
| 3.4.21.22 | lithium ion | activates slightly | Homo sapiens | |
| 3.4.21.22 | Na+ | activates the amidolytic activity of the enzyme, but does only marginally influence the proteolytic activity of factor IXa in the prothrombinase complex, not required for activation of factor X | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.22 | factor X + H2O | Homo sapiens | proteolytic activity, factor IXa acts as part of phosphocholine/phosphoserine vesicles interacting with factor VIIIa in the complex in a sodium-dependent manner, overview | activated factor X + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.22 | Homo sapiens | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.22 | commercial preparation | from plasma | Homo sapiens | - |
| 3.4.21.22 | plasma | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.22 | additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.22 | CH3SO2-D-Leu-Gly-Arg-4-nitroanilide + H2O | amidolytic activity | Homo sapiens | ? | - |
? | |
| 3.4.21.22 | factor X + H2O | proteolytic activity, factor IXa acts as part of phosphocholine/phosphoserine vesicles interacting with factor VIIIa in the complex in a sodium-dependent manner, overview | Homo sapiens | activated factor X + ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.22 | factor IXa | - |
Homo sapiens |
| 3.4.21.22 | FIXa | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.22 | 22 | - |
assay at room temperature | Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.22 | 18.5 | - |
CH3SO2-D-Leu-Gly-Arg-4-nitroanilide | pH 7.5, 22°C, in presence of 5 mM Ca2+ | Homo sapiens | |
| 3.4.21.22 | 19 | - |
CH3SO2-D-Leu-Gly-Arg-4-nitroanilide | pH 7.5, 22°C, in presence of 5 mM Ca2+ and 0.2 M LiCl | Homo sapiens | |
| 3.4.21.22 | 20.2 | - |
CH3SO2-D-Leu-Gly-Arg-4-nitroanilide | pH 7.5, 22°C, in presence of 5 mM Ca2+ and 0.2 M NaCl | Homo sapiens | |
| 3.4.21.22 | 21.3 | - |
CH3SO2-D-Leu-Gly-Arg-4-nitroanilide | pH 7.5, 22°C, in presence of 5 mM Ca2+ and 0.2 M ChCl | Homo sapiens | |
| 3.4.21.22 | 21.9 | - |
CH3SO2-D-Leu-Gly-Arg-4-nitroanilide | pH 7.5, 22°C, in presence of 5 mM Ca2+ and 0.2 M RbCl | Homo sapiens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.22 | 7.5 | - |
assay at | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
