Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Marienhagen, J.; Sandalova, T.; Sahm, H.; Eggeling, L.; Schneider, G.
    Insights into the structural basis of substrate recognition by histidinol-phosphate aminotransferase from Corynebacterium glutamicum (2008), Acta Crystallogr. Sect. D, 64, 675-685.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.9 apo histidinol-phosphate aminotransferase the internal PLP aldimine adduct and a pyridoxamine 5'-phosphate enzyme complex at resolutions of 2.2 A, 2.1 A and 1.8 A, respectively. The hydrogen bond between the side chain of residue Y21 and the phosphate group of histidinol phosphate is important for recognition of the natural substrate and discrimination against other potential amino donors. Residue N99 does not contribute to the specific recognition of the amino-acid donor. Residues Y123 and Y257 interact with the substrate through van der Waals-interactions Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.9 N99D 6fold reduction in ratio kcat to Km for substrates L-histidinol phosphate, 2-oxoglutarate Corynebacterium glutamicum
2.6.1.9 Y123F 4fold reduction in ratio kcat to Km for substrates L-histidinol phosphate, 2-oxoglutarate Corynebacterium glutamicum
2.6.1.9 Y21F 10fold reduction in ratio kcat to Km for substrates L-histidinol phosphate, 2-oxoglutarate Corynebacterium glutamicum
2.6.1.9 Y257F 5fold reduction in ratio kcat to Km for substrates L-histidinol phosphate, 2-oxoglutarate Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.9 0.89
-
 L-histidinol phosphate wild-type Corynebacterium glutamicum
2.6.1.9 1.3
-
 L-histidinol phosphate mutant Y123F Corynebacterium glutamicum
2.6.1.9 2.2
-
 L-histidinol phosphate mutant Y257F Corynebacterium glutamicum
2.6.1.9 6.7
-
 L-histidinol phosphate mutant Y21F Corynebacterium glutamicum
2.6.1.9 9.1
-
 L-histidinol phosphate mutant N99D Corynebacterium glutamicum
2.6.1.9 22.2
-
 L-phenylalanine mutant Y257F Corynebacterium glutamicum
2.6.1.9 25.5
-
 L-phenylalanine mutant Y21F Corynebacterium glutamicum
2.6.1.9 27.5
-
 L-phenylalanine mutant Y123F Corynebacterium glutamicum
2.6.1.9 32.8
-
 L-leucine mutant Y257F Corynebacterium glutamicum
2.6.1.9 53.4
-
 L-leucine mutant Y123F Corynebacterium glutamicum
2.6.1.9 55.8
-
 L-leucine mutant N99D Corynebacterium glutamicum
2.6.1.9 71.4
-
 L-phenylalanine mutant N99D Corynebacterium glutamicum
2.6.1.9 80.8
-
 L-leucine mutant Y21F Corynebacterium glutamicum
2.6.1.9 94.9
-
 L-leucine wild-type Corynebacterium glutamicum
2.6.1.9 106
-
 L-phenylalanine wild-type Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.9 Corynebacterium glutamicum Q9KJU4
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.9 0.1
-
 substrates 4-methyl-2-oxopentanoate, L-alanine Corynebacterium glutamicum
2.6.1.9 0.2
-
 substrates L-glutarate, 2-oxobutanoate Corynebacterium glutamicum
2.6.1.9 0.3
-
 substrates 3-indole-2-oxopropanoate, L-glutamate Corynebacterium glutamicum
2.6.1.9 0.4
-
 substrates 4-methyl-2-oxopentanoate, L-asparagine Corynebacterium glutamicum
2.6.1.9 0.5
-
 substrates 3-(4-hydroxyphenyl)-2-oxopropanoate, L-glutamate Corynebacterium glutamicum
2.6.1.9 1.4
-
 substrates 4-methyl-2-oxopentanoate, L-glutamate Corynebacterium glutamicum
2.6.1.9 1.5
-
 substrates phenylpyruvate, L-glutamate Corynebacterium glutamicum
2.6.1.9 2.8
-
 substrates L-histidinol phosphate, 2-oxoglutarate Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.9 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 Corynebacterium glutamicum L-tyrosine + 2-oxoglutarate
-
 ?
2.6.1.9 3-indole-2-oxopropanoate + L-glutamate
-
 Corynebacterium glutamicum L-tryptophan + 2-oxoglutarate
-
 ?
2.6.1.9 4-methyl-2-oxopentanoate + L-alanine
-
 Corynebacterium glutamicum L-leucine + pyruvate
-
 ?
2.6.1.9 4-methyl-2-oxopentanoate + L-asparagine
-
 Corynebacterium glutamicum L-leucine + 2-oxosuccinamic acid
-
 ?
2.6.1.9 4-methyl-2-oxopentanoate + L-glutamate
-
 Corynebacterium glutamicum L-leucine + 2-oxoglutarate
-
 ?
2.6.1.9 L-glutarate + 2-oxobutanoate
-
 Corynebacterium glutamicum 2-oxoglutarate + 2-aminobutanoate
-
 ?
2.6.1.9 L-histidinol phosphate + 2-oxoglutarate
-
 Corynebacterium glutamicum 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
-
 ?
2.6.1.9 additional information no substrate: 3-methyl-2-oxopentanoate, 3-methyl-2-oxobutanoate Corynebacterium glutamicum ?
-
 ?
2.6.1.9 phenylpyruvate + L-glutamate
-
 Corynebacterium glutamicum L-phenylalanine + 2-oxoglutarate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.9 0.39
-
 L-histidinol phosphate mutant Y123F Corynebacterium glutamicum
2.6.1.9 0.51
-
 L-histidinol phosphate mutant Y257F Corynebacterium glutamicum
2.6.1.9 0.59
-
 L-phenylalanine mutant Y123F Corynebacterium glutamicum
2.6.1.9 0.66
-
 L-leucine mutant Y123F Corynebacterium glutamicum
2.6.1.9 0.68
-
 L-phenylalanine mutant Y257F Corynebacterium glutamicum
2.6.1.9 0.69
-
 L-leucine mutant Y257F Corynebacterium glutamicum
2.6.1.9 0.87
-
 L-phenylalanine mutant Y21F Corynebacterium glutamicum
2.6.1.9 1.18
-
 L-histidinol phosphate wild-type Corynebacterium glutamicum
2.6.1.9 1.6
-
 L-leucine mutant N99D Corynebacterium glutamicum
2.6.1.9 1.72
-
 L-leucine mutant Y21F Corynebacterium glutamicum
2.6.1.9 1.86
-
 L-histidinol phosphate mutant N99D Corynebacterium glutamicum
2.6.1.9 1.93
-
 L-histidinol phosphate mutant Y21F Corynebacterium glutamicum
2.6.1.9 2.22
-
 L-phenylalanine mutant N99D Corynebacterium glutamicum
2.6.1.9 3.27
-
 L-leucine wild-type Corynebacterium glutamicum
2.6.1.9 4.1
-
 L-phenylalanine wild-type Corynebacterium glutamicum