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Literature summary extracted from

  • Muraki, N.; Seo, D.; Shiba, T.; Sakurai, T.; Kurisu, G.
    Crystallization and preliminary X-ray studies of ferredoxin-NAD(P)+ reductase from Chlorobium tepidum (2008), Acta Crystallogr. Sect. F, 64, 186-189.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.2 expressed in Escherichia coli Tuner(DE3)pLacI cells Chlorobaculum tepidum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.18.1.2 hanging drop vapour diffusion method, using 0.1 M MES buffer pH 5.8 containing 16% (w/v) PEG 6K and 0.1 M ammonium sulfate as precipitant Chlorobaculum tepidum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.2 45000
-
 2 * 45000, gel filtration Chlorobaculum tepidum
1.18.1.2 90000
-
 gel filtration Chlorobaculum tepidum

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Chlorobaculum tepidum
-
-
-
1.18.1.2 Chlorobaculum tepidum CT1512
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2 ammonium sulfate precipitation, Matrex Red A column chromatography, DEAE-5PW column chromatography, hydroxyapatite column chromatography, and Superdex-200 gel filtration Chlorobaculum tepidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 reduced ferredoxin + NAD(P)+
-
 Chlorobaculum tepidum oxidized ferredoxin + NAD(P)H
-
 r
1.18.1.2 reduced ferredoxin + NAD(P)+
-
 Chlorobaculum tepidum CT1512 oxidized ferredoxin + NAD(P)H
-
 r

Subunits

EC Number Subunits Comment Organism
1.18.1.2 homodimer 2 * 45000, gel filtration Chlorobaculum tepidum

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 Fd-NADP+ reductase
-
 Chlorobaculum tepidum
1.18.1.2 Ferredoxin-NADP(+) reductase
-
 Chlorobaculum tepidum
1.18.1.2 FNR
-
 Chlorobaculum tepidum

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.2 Ferredoxin
-
 Chlorobaculum tepidum
1.18.1.2 NAD(P)+
-
 Chlorobaculum tepidum