BRENDA - Enzyme Database show

Purification and biochemical characterization of a novel glutathione S-transferase of the silkworm, Bombyx mori

Hou, C.X.; Fu, Z.Q.; Jin, B.R.; Gui, Z.Z.; Afr. J. Biotechnol. 7, 311-316 (2008)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.5.1.18
albendazol
17.87% inhibition at 1 mM
Bombyx mori
2.5.1.18
bile acid
41.78% inhibition at 1 mM
Bombyx mori
2.5.1.18
NaCl
26.22% inhibition at 1 mM
Bombyx mori
2.5.1.18
praziquantel
31.31% inhibition at 1 mM
Bombyx mori
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.18
0.49
-
glutathione
at 25C
Bombyx mori
2.5.1.18
0.494
-
1-chloro-2,4-dinitrobenzene
at 25C
Bombyx mori
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.18
28000
-
SDS-PAGE
Bombyx mori
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.5.1.18
Bombyx mori
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.5.1.18
glutathione agarose column chromatography
Bombyx mori
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.5.1.18
0.728
-
crude extract, at 25C
Bombyx mori
2.5.1.18
4610
-
after 6332fold purification, at 25C
Bombyx mori
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.18
glutathione + 1-chloro-2,4-dinitrobenzene
-
684256
Bombyx mori
S-(2,4-dinitrophenyl)-glutathione + HCl
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.5.1.18
25
-
-
Bombyx mori
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.5.1.18
15
70
at temperatures ranging from 15C to 70C, the enzymatic activity increases with increasing temperature up to 25C, then the activity starts to decrease
Bombyx mori
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.5.1.18
57.87
-
glutathione
at 25C
Bombyx mori
2.5.1.18
65.43
-
1-chloro-2,4-dinitrobenzene
at 25C
Bombyx mori
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.18
7.1
-
-
Bombyx mori
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.5.1.18
5.1
9
at pH values ranging from 5.4-7.1, the enzymatic activity increases with increasing pH, then the activity starts to decrease
Bombyx mori
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.5.1.18
albendazol
17.87% inhibition at 1 mM
Bombyx mori
2.5.1.18
bile acid
41.78% inhibition at 1 mM
Bombyx mori
2.5.1.18
NaCl
26.22% inhibition at 1 mM
Bombyx mori
2.5.1.18
praziquantel
31.31% inhibition at 1 mM
Bombyx mori
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.18
0.49
-
glutathione
at 25C
Bombyx mori
2.5.1.18
0.494
-
1-chloro-2,4-dinitrobenzene
at 25C
Bombyx mori
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.18
28000
-
SDS-PAGE
Bombyx mori
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.18
glutathione agarose column chromatography
Bombyx mori
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.5.1.18
0.728
-
crude extract, at 25C
Bombyx mori
2.5.1.18
4610
-
after 6332fold purification, at 25C
Bombyx mori
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.18
glutathione + 1-chloro-2,4-dinitrobenzene
-
684256
Bombyx mori
S-(2,4-dinitrophenyl)-glutathione + HCl
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.5.1.18
25
-
-
Bombyx mori
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.5.1.18
15
70
at temperatures ranging from 15C to 70C, the enzymatic activity increases with increasing temperature up to 25C, then the activity starts to decrease
Bombyx mori
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.5.1.18
57.87
-
glutathione
at 25C
Bombyx mori
2.5.1.18
65.43
-
1-chloro-2,4-dinitrobenzene
at 25C
Bombyx mori
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.18
7.1
-
-
Bombyx mori
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.5.1.18
5.1
9
at pH values ranging from 5.4-7.1, the enzymatic activity increases with increasing pH, then the activity starts to decrease
Bombyx mori