BRENDA - Reference Id = 684256

Purification and biochemical characterization of a novel glutathione S-transferase of the silkworm, Bombyx mori

Hou, C.X.; Fu, Z.Q.; Jin, B.R.; Gui, Z.Z.; Afr. J. Biotechnol. 7, 311-316 (2008)

No PubMed abstract available

Data extracted from this reference:

Inhibitors

2.5.1.18

albendazol

17.87% inhibition at 1 mM

Bombyx mori

2.5.1.18

bile acid

41.78% inhibition at 1 mM

Bombyx mori

2.5.1.18

NaCl

26.22% inhibition at 1 mM

Bombyx mori

2.5.1.18

praziquantel

31.31% inhibition at 1 mM

Bombyx mori

KM Value [mM]

2.5.1.18

0.49

glutathione

at 25°C

Bombyx mori

2.5.1.18

0.494

1-chloro-2,4-dinitrobenzene

at 25°C

Bombyx mori

Molecular Weight [Da]

2.5.1.18

28000

SDS-PAGE

Bombyx mori

Organism

2.5.1.18

Bombyx mori

Purification (Commentary)

2.5.1.18

glutathione agarose column chromatography

Bombyx mori

Specific Activity [micromol/min/mg]

2.5.1.18

0.728

crude extract, at 25°C

Bombyx mori

2.5.1.18

4610

after 6332fold purification, at 25°C

Bombyx mori

Substrates and Products (Substrate)

2.5.1.18

glutathione + 1-chloro-2,4-dinitrobenzene

684256

Bombyx mori

S-(2,4-dinitrophenyl)-glutathione + HCl

Temperature Optimum [°C]

2.5.1.18

Bombyx mori

Temperature Stability [°C]

2.5.1.18

at temperatures ranging from 15°C to 70°C, the enzymatic activity increases with increasing temperature up to 25°C, then the activity starts to decrease

Bombyx mori

Turnover Number [1/s]

2.5.1.18

57.87

glutathione

at 25°C

Bombyx mori

2.5.1.18

65.43

1-chloro-2,4-dinitrobenzene

at 25°C

Bombyx mori

pH Optimum

2.5.1.18

7.1

Bombyx mori

pH Stability

2.5.1.18

5.1

at pH values ranging from 5.4-7.1, the enzymatic activity increases with increasing pH, then the activity starts to decrease

Bombyx mori

Inhibitors (protein specific)

2.5.1.18

albendazol

17.87% inhibition at 1 mM

Bombyx mori

2.5.1.18

bile acid

41.78% inhibition at 1 mM

Bombyx mori

2.5.1.18

NaCl

26.22% inhibition at 1 mM

Bombyx mori

2.5.1.18

praziquantel

31.31% inhibition at 1 mM

Bombyx mori

KM Value [mM] (protein specific)

2.5.1.18

0.49

glutathione

at 25°C

Bombyx mori

2.5.1.18

0.494

1-chloro-2,4-dinitrobenzene

at 25°C

Bombyx mori

Molecular Weight [Da] (protein specific)

2.5.1.18

28000

SDS-PAGE

Bombyx mori

Purification (Commentary) (protein specific)

2.5.1.18

glutathione agarose column chromatography

Bombyx mori

Specific Activity [micromol/min/mg] (protein specific)

2.5.1.18

0.728

crude extract, at 25°C

Bombyx mori

2.5.1.18

4610

after 6332fold purification, at 25°C

Bombyx mori

Substrates and Products (Substrate) (protein specific)

2.5.1.18

glutathione + 1-chloro-2,4-dinitrobenzene

684256

Bombyx mori

S-(2,4-dinitrophenyl)-glutathione + HCl

Temperature Optimum [°C] (protein specific)

2.5.1.18

Bombyx mori

Temperature Stability [°C] (protein specific)

2.5.1.18

at temperatures ranging from 15°C to 70°C, the enzymatic activity increases with increasing temperature up to 25°C, then the activity starts to decrease

Bombyx mori

Turnover Number [1/s] (protein specific)

2.5.1.18

57.87

glutathione

at 25°C

Bombyx mori

2.5.1.18

65.43

1-chloro-2,4-dinitrobenzene

at 25°C

Bombyx mori

pH Optimum (protein specific)

2.5.1.18

7.1

Bombyx mori

pH Stability (protein specific)

2.5.1.18

5.1

at pH values ranging from 5.4-7.1, the enzymatic activity increases with increasing pH, then the activity starts to decrease

Bombyx mori