BRENDA - Enzyme Database

Purification and biochemical characterization of a novel glutathione S-transferase of the silkworm, Bombyx mori

Hou, C.X.; Fu, Z.Q.; Jin, B.R.; Gui, Z.Z.; Afr. J. Biotechnol. 7, 311-316 (2008)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.5.1.18
albendazol
17.87% inhibition at 1 mM
Bombyx mori
2.5.1.18
bile acid
41.78% inhibition at 1 mM
Bombyx mori
2.5.1.18
NaCl
26.22% inhibition at 1 mM
Bombyx mori
2.5.1.18
praziquantel
31.31% inhibition at 1 mM
Bombyx mori
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.18
0.49
-
glutathione
at 25°C
Bombyx mori
2.5.1.18
0.494
-
1-chloro-2,4-dinitrobenzene
at 25°C
Bombyx mori
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.18
28000
-
SDS-PAGE
Bombyx mori
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.5.1.18
Bombyx mori
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.5.1.18
glutathione agarose column chromatography
Bombyx mori
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.5.1.18
0.728
-
crude extract, at 25°C
Bombyx mori
2.5.1.18
4610
-
after 6332fold purification, at 25°C
Bombyx mori
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.18
glutathione + 1-chloro-2,4-dinitrobenzene
-
684256
Bombyx mori
S-(2,4-dinitrophenyl)-glutathione + HCl
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.5.1.18
25
-
-
Bombyx mori
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.5.1.18
15
70
at temperatures ranging from 15°C to 70°C, the enzymatic activity increases with increasing temperature up to 25°C, then the activity starts to decrease
Bombyx mori
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.5.1.18
57.87
-
glutathione
at 25°C
Bombyx mori
2.5.1.18
65.43
-
1-chloro-2,4-dinitrobenzene
at 25°C
Bombyx mori
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.18
7.1
-
-
Bombyx mori
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.5.1.18
5.1
9
at pH values ranging from 5.4-7.1, the enzymatic activity increases with increasing pH, then the activity starts to decrease
Bombyx mori
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.5.1.18
albendazol
17.87% inhibition at 1 mM
Bombyx mori
2.5.1.18
bile acid
41.78% inhibition at 1 mM
Bombyx mori
2.5.1.18
NaCl
26.22% inhibition at 1 mM
Bombyx mori
2.5.1.18
praziquantel
31.31% inhibition at 1 mM
Bombyx mori
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.18
0.49
-
glutathione
at 25°C
Bombyx mori
2.5.1.18
0.494
-
1-chloro-2,4-dinitrobenzene
at 25°C
Bombyx mori
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.18
28000
-
SDS-PAGE
Bombyx mori
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.18
glutathione agarose column chromatography
Bombyx mori
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.5.1.18
0.728
-
crude extract, at 25°C
Bombyx mori
2.5.1.18
4610
-
after 6332fold purification, at 25°C
Bombyx mori
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.18
glutathione + 1-chloro-2,4-dinitrobenzene
-
684256
Bombyx mori
S-(2,4-dinitrophenyl)-glutathione + HCl
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.5.1.18
25
-
-
Bombyx mori
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.5.1.18
15
70
at temperatures ranging from 15°C to 70°C, the enzymatic activity increases with increasing temperature up to 25°C, then the activity starts to decrease
Bombyx mori
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.5.1.18
57.87
-
glutathione
at 25°C
Bombyx mori
2.5.1.18
65.43
-
1-chloro-2,4-dinitrobenzene
at 25°C
Bombyx mori
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.18
7.1
-
-
Bombyx mori
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.5.1.18
5.1
9
at pH values ranging from 5.4-7.1, the enzymatic activity increases with increasing pH, then the activity starts to decrease
Bombyx mori