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Literature summary extracted from

  • Sullivan, R.; Zhao, H.
    Cloning, characterization, and mutational analysis of a highly active and stable L-arabinitol 4-dehydrogenase from Neurospora crassa (2007), Appl. Microbiol. Biotechnol., 77, 845-852.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.12 analysis enzymatic cycling assay for nicotinic acid adenine dinucleotide phosphate Neurospora crassa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.12 expression in Escherichia coli Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.12 18
-
 L-arabinitol pH 8.0, 25°C Neurospora crassa
1.1.1.12 35
-
 adonitol pH 8.0, 25°C Neurospora crassa
1.1.1.12 174
-
 NAD+ pH 8.0, 25°C Neurospora crassa
1.1.1.12 290
-
 xylitol pH 8.0, 25°C Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.12 Zn2+ two ions per subunit Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.12 39000
-
 4 * 39000, SDS-PAGE, 4 * 39600, calculated Neurospora crassa
1.1.1.12 39600
-
 4 * 39000, SDS-PAGE, 4 * 39600, calculated Neurospora crassa
1.1.1.12 152000
-
 gel filtration Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.12 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.12 recombinant protein Neurospora crassa

Storage Stability

EC Number Storage Stability Organism
1.1.1.12 22°C, stable for more than 1 month Neurospora crassa
1.1.1.12 4°C, stable for several months Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.12 adonitol + NAD+
-
 Neurospora crassa ? + NADH
-
 ?
1.1.1.12 L-arabinitol + NAD+
-
 Neurospora crassa L-xylulose + NADH + H+
-
 ?
1.1.1.12 additional information no substrate: D-mannitol, D-arabinitol, D-sorbitol Neurospora crassa ?
-
 ?
1.1.1.12 Xylitol + NAD+
-
 Neurospora crassa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.12 tetramer 4 * 39000, SDS-PAGE, 4 * 39600, calculated Neurospora crassa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.12 45 55
-
 Neurospora crassa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.12 45
-
 4 h, more than 60% residual activity Neurospora crassa
1.1.1.12 50
-
 half-life 45 min Neurospora crassa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.12 15
-
 xylitol pH 8.0, 25°C Neurospora crassa
1.1.1.12 18
-
 adonitol pH 8.0, 25°C Neurospora crassa
1.1.1.12 20.1
-
 NAD+ pH 8.0, 25°C Neurospora crassa
1.1.1.12 20.2
-
 L-arabinitol pH 8.0, 25°C Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.12 9.5
-
-
 Neurospora crassa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.12 8 10.5 more than 60% of maximal activity within Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.12 NAD+ strong preference for NAD+ Neurospora crassa
1.1.1.12 NADH
-
 Neurospora crassa
1.1.1.12 NADP+ very low yet detectable activity with NADP+, strong preference for NAD+ Neurospora crassa
1.1.1.12 NADPH
-
 Neurospora crassa