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Literature summary extracted from

  • Akhtar, M.K.; Jones, P.R.
    Deletion of iscR stimulates recombinant clostridial Fe-Fe hydrogenase activity and H2-accumulation in Escherichia coli BL21(DE3) (2008), Appl. Microbiol. Biotechnol., 78, 853-862.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.7.2 expressed in Escherichia coli BL21(DE3) cells Clostridium acetobutylicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.7.2 Iron one mol of HydA contains 7 mol iron Clostridium acetobutylicum

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Clostridium acetobutylicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2 Ni-Sepharose 6 Fast Flow chromatography Clostridium acetobutylicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.7.2 28
-
 recombinant, purified enzyme Clostridium acetobutylicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H2 + oxidized ferredoxin under anoxic conditions Clostridium acetobutylicum H+ + reduced ferredoxin
-
 r
1.12.7.2 H2 + oxidized methyl viologen
-
 Clostridium acetobutylicum H+ + reduced methyl viologen
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.12.7.2 Fe-Fe hydrogenase
-
 Clostridium acetobutylicum
1.12.7.2 hydA
-
 Clostridium acetobutylicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.7.2 Ferredoxin dependent Clostridium acetobutylicum
1.12.7.2 iron-sulfur centre
-
 Clostridium acetobutylicum