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Literature summary extracted from
- Biochemical characterization of isocitrate dehydrogenase from Methylococcus capsulatus reveals a unique NAD+-dependent homotetrameric enzyme (2007), Arch. Microbiol., 187, 361-370.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.41 | cloned and overexpressed in Escherichia coli | Methylococcus capsulatus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.41 | 0.0517 | - |
isocitrate | - |
Methylococcus capsulatus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.41 | 36340 | - |
4 * 36340, the clasp-like domain of McIDH is a likely site for tetramerization, calculated from sequence | Methylococcus capsulatus |
| 1.1.1.41 | 145000 | - |
gel filtration | Methylococcus capsulatus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.41 | Methylococcus capsulatus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.41 | - |
Methylococcus capsulatus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.41 | isocitrate + NAD+ | the enzyme is NAD+-dependent | Methylococcus capsulatus | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.41 | tetramer | 4 * 36340, the clasp-like domain of McIDH is a likely site for tetramerization, calculated from sequence | Methylococcus capsulatus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.41 | McIDH | - |
Methylococcus capsulatus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.41 | 55 | 60 | - |
Methylococcus capsulatus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.41 | 70 | - |
apparent midpoint melting temperature | Methylococcus capsulatus |
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Release 2023.1 (January 2023)
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