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Literature summary extracted from

  • Annoura, T.; Sariego, I.; Nara, T.; Makiuchi, T.; Fujimura, T.; Taka, H.; Mineki, R.; Murayama, K.; Aoki, T.
    Dihydroorotate dehydrogenase arises from novel fused gene product with aspartate carbamoyltransferase in Bodo saliens (2007), Biochem. Biophys. Res. Commun., 358, 253-258.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.98.1 35000
-
 mature enzyme, SDS-PAGE Neobodo saliens
1.3.98.1 70000
-
 premature enzyme fused to aspartate carbamoyltransferase, SDS-PAGE Neobodo saliens
2.1.3.2 35000
-
 x * 35000, SDS-PAGE Neobodo saliens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.2 additional information Neobodo saliens ACT-DHOD gene is transcribed to ACT-DHOD mRNA, translated to the single protein, ACT-DHOD, and finally converted to mature independent DHOD and ACT ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.98.1 Neobodo saliens
-
-
-
2.1.3.2 Neobodo saliens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.98.1 the enzyme is fused with aspartate carbamoyltransferase as precursor to mature DHOD Neobodo saliens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.98.1 0.987
-
 using 0.02 mM ubiquinone-1 as acceptor, at 25°C Neobodo saliens
1.3.98.1 3.318
-
 using 0.5 mM fumarate as acceptor, at 25°C Neobodo saliens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.98.1 dihydroorotate + fumarate prefers fumarate over ubiquinone-1 Neobodo saliens orotate + succinate
-
 ?
1.3.98.1 dihydroorotate + ubiquinone-1
-
 Neobodo saliens orotate + ubiquinol-1
-
 ?
2.1.3.2 additional information ACT-DHOD gene is transcribed to ACT-DHOD mRNA, translated to the single protein, ACT-DHOD, and finally converted to mature independent DHOD and ACT Neobodo saliens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.2 ? x * 35000, SDS-PAGE Neobodo saliens

Synonyms

EC Number Synonyms Comment Organism
1.3.98.1 DHOD
-
 Neobodo saliens
2.1.3.2 ACT
-
 Neobodo saliens