EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.9 | thiamine diphosphate | required, Km: 0.0024 mM | Escherichia coli |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.2.1.9 | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.2.1.9 | 0.053 | - |
isochorismate | pH 7.8, 22°C | Escherichia coli | |
2.2.1.9 | 1.5 | - |
2-oxoglutarate | pH 7.8, 22°C | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.9 | Mg2+ | required, Km: 0.08 mM | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.2.1.9 | isochorismate + 2-oxoglutarate | Escherichia coli | the enzyme is involved in biosynthesis of vitamin K2 (menoquinone). Under basic conditions, the product can spontaneously lose pyruvate to form (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate | 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2 | - |
? | |
4.2.99.20 | 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate | Escherichia coli K-12 | the enzyme is involved in biosynthesis of vitamin K2 (manoquinone). Under basic conditions, the product can spontaneously lose pyruvate to form (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate | (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.2.1.9 | Escherichia coli | - |
- |
- |
4.2.99.20 | Escherichia coli K-12 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.2.1.9 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.2.1.9 | isochorismate + 2-oxoglutarate | the enzyme is involved in biosynthesis of vitamin K2 (menoquinone). Under basic conditions, the product can spontaneously lose pyruvate to form (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate | Escherichia coli | 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2 | - |
? | |
2.2.1.9 | isochorismate + 2-oxoglutarate | under basic conditions, the product can spontaneously lose pyruvate to form (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate | Escherichia coli | 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2 | - |
? | |
4.2.99.20 | 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate | - |
Escherichia coli K-12 | (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate | - |
? | |
4.2.99.20 | 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate | the enzyme is involved in biosynthesis of vitamin K2 (manoquinone). Under basic conditions, the product can spontaneously lose pyruvate to form (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate | Escherichia coli K-12 | (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.2.1.9 | MenD | - |
Escherichia coli |
2.2.1.9 | SEPHCHC synthase | - |
Escherichia coli |
4.2.99.20 | SHCHC synthase | - |
Escherichia coli K-12 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.2.1.9 | 0.05 | - |
isochorismate | pH 7.8, 22°C | Escherichia coli | |
2.2.1.9 | 0.075 | - |
2-oxoglutarate | pH 7.8, 22°C | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.2.1.9 | 7 | 8 | phosphate buffer | Escherichia coli |