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Literature summary extracted from

  • Vashishtha, A.K.; West, A.H.; Cook, P.F.
    Overall kinetic mechanism of saccharopine dehydrogenase (L-glutamate forming) from Saccharomyces cerevisiae (2008), Biochemistry, 47, 5417-5423.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.10 expressed in Escherichia coli BL21/(DE-3) RIL cells Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.10 2-amino-6-heptenoic acid competitive inhibition versus L-alpha-aminoadipate-delta-semialdehyde, uncompetitive inhibition versus NADPH, and noncompetitive inhibitition versus L-glutamate Saccharomyces cerevisiae
1.5.1.10 2-oxoglutarate competitive inhibition versus L-glutamate and uncompetitive inhibition versus L-alpha-aminoadipate-delta-semialdehyde and NADPH; competitive inhibition versus saccharopine and uncompetitive inhibition versus NADP+ Saccharomyces cerevisiae
1.5.1.10 alpha-AASA shows noncompetitive inhibition versus saccharopine and uncompetitive inhibition versus NADP+ Saccharomyces cerevisiae
1.5.1.10 glyoxylic acid competitive inhibition versus saccharopine and uncompetitive inhibition versus NADP+ Saccharomyces cerevisiae
1.5.1.10 L-glutamate exhibits noncompetitive inhibition versus NADP+ and saccharopine Saccharomyces cerevisiae
1.5.1.10 L-leucine competitive inhibition versus saccharopine and uncompetitive inhibition versus NADP+ Saccharomyces cerevisiae
1.5.1.10 L-ornithine competitive inhibition versus saccharopine and uncompetitive inhibition versus NADP+ Saccharomyces cerevisiae
1.5.1.10 L-pipecolic acid competitive inhibition versus saccharopine and uncompetitive inhibition versus NADP+ Saccharomyces cerevisiae
1.5.1.10 N-oxalylglycine competitive inhibition versus saccharopine and uncompetitive inhibition versus NADP+ Saccharomyces cerevisiae
1.5.1.10 NADP+ competitive inhibition versus NADPH, noncompetitive inhibition versus L-alpha-aminoadipate-delta-semialdehyde and L-glutamate Saccharomyces cerevisiae
1.5.1.10 NADPH inhibition is competitive versus NADP+ and noncompetitive versus saccharopine Saccharomyces cerevisiae
1.5.1.10 saccharopine exhibits noncompetitive inhibition against L-alpha-aminoadipate-delta-semialdehyde, L-glutamate, and NADPH Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.10 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.10 Ni-NTA column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.10 2-aminoadipate 6-semialdehyde + L-glutamate + NADPH + H+
-
 Saccharomyces cerevisiae N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.5.1.10 saccharopine dehydrogenase (L-glutamate forming)
-
 Saccharomyces cerevisiae
1.5.1.10 saccharopine reductase
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.10 7
-
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.10 NADPH
-
 Saccharomyces cerevisiae