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Literature summary extracted from

  • Zhao, G.; Bruckner, R.C.; Jorns, M.S.
    Identification of the oxygen activation site in monomeric sarcosine oxidase: role of lys265 in catalysis (2008), Biochemistry, 47, 9124-9135.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.1 mutant proteins expressed as His-tag fusion proteins in Escherichia coli BL21(DE3) Bacillus sp. (in: Bacteria)

Protein Variants

EC Number Protein Variants Comment Organism
1.5.3.1 K265A only traces of activity Bacillus sp. (in: Bacteria)
1.5.3.1 K265M only traces of activity Bacillus sp. (in: Bacteria)
1.5.3.1 K265Q only traces of activity Bacillus sp. (in: Bacteria)
1.5.3.1 K265R less than 30% of covalently bound FAD, strongly reduced turnover Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.1 methylthioacetate competitive Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.1 44000
-
 1 * 44000 Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.1 sarcosine + O2 + H2O Bacillus sp. (in: Bacteria)
-
 formaldehyde + glycine + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.1 Bacillus sp. (in: Bacteria)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.1 recombinant proteins using His-tag Bacillus sp. (in: Bacteria)

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.3.1 sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 mechanism suggested Bacillus sp. (in: Bacteria)
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.1 sarcosine + O2 + H2O
-
 Bacillus sp. (in: Bacteria) formaldehyde + glycine + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.1 monomer 1 * 44000 Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
1.5.3.1 monomeric sarcosine oxidase
-
 Bacillus sp. (in: Bacteria)
1.5.3.1 MSOX
-
 Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.1 0.0049
-
 sarcosine K265Q mutant, pH 8.0, 25°C Bacillus sp. (in: Bacteria)
1.5.3.1 0.0066
-
 sarcosine K265A mutant, pH 8.0, 25°C Bacillus sp. (in: Bacteria)
1.5.3.1 0.0076
-
 sarcosine K265M mutant, pH 8.0, 25°C Bacillus sp. (in: Bacteria)
1.5.3.1 0.272
-
 sarcosine K265R mutant, pH 8.0, 25°C Bacillus sp. (in: Bacteria)
1.5.3.1 45.8
-
 sarcosine native protein, pH 8.0, 25°C Bacillus sp. (in: Bacteria)

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.1 FAD covalently bound Bacillus sp. (in: Bacteria)