Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Boselli, A.; Rosini, E.; Marcone, G.L.; Sacchi, S.; Motteran, L.; Pilone, M.S.; Pollegioni, L.; Molla, G.
    Glycine oxidase from Bacillus subtilis: role of histidine 244 and methionine 261 (2007), Biochimie, 89, 1372-1380.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.19 expressed in Escherichia coli strain BL21(DE3)pLysS Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.19 H244F shows increased kcat value for glycine compared to the wild type enzyme, the mutation does not affect the expression of glycine oxidase and the physicochemical properties of bound FAD Bacillus subtilis
1.4.3.19 H244N shows increased kcat value for glycine compared to the wild type enzyme, the mutation does not affect the expression of glycine oxidase and the physicochemical properties of bound FAD Bacillus subtilis
1.4.3.19 H244Q shows increased kcat value compared to the wild type enzyme, the mutation does not affect the expression of glycine oxidase and the physicochemical properties of bound FAD Bacillus subtilis
1.4.3.19 M261H shows decreased kcat value for glycine compared to the wild type enzyme, the mutation does not affect the expression of glycine oxidase and the physicochemical properties of bound FAD Bacillus subtilis
1.4.3.19 M261Y shows decreased kcat value for glycine compared to the wild type enzyme, the mutation does not affect the expression of glycine oxidase and the physicochemical properties of bound FAD Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.19 0.6
-
 glycine apparent value for mutant enzyme H244F, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 0.7
-
 glycine apparent value for wild type enzyme, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 0.7
-
 sarcosine apparent value for wild type enzyme, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 0.8
-
 glycine apparent value for mutant enzyme H244Q, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 1.4
-
 sarcosine apparent value for mutant enzyme H244F, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 1.9
-
 glycine apparent value for mutant enzyme H244N, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 2.1
-
 sarcosine apparent value for mutant enzyme H244N, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 2.8
-
 sarcosine apparent value for mutant enzyme H244Q, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 4 10 D-alanine apparent value for mutant enzyme M261Y, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 11
-
 sarcosine apparent value for mutant enzyme M261H, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 11
-
 sarcosine apparent value for mutant enzyme M261Y, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 17
-
 D-proline apparent value for wild type enzyme, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 18
-
 glycine apparent value for mutant enzyme M261Y, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 26
-
 D-proline apparent value for mutant enzyme H244F, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 26
-
 D-proline apparent value for mutant enzyme H244N, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 27
-
 D-proline apparent value for mutant enzyme H244Q, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 28
-
 glycine apparent value for mutant enzyme M261H, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 280
-
 D-proline apparent value for mutant enzyme M261H, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 285
-
 D-proline apparent value for mutant enzyme M261Y, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 350
-
 D-alanine apparent value for mutant enzyme H244Q, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 420
-
 D-alanine apparent value for mutant enzyme H244N, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 490
-
 D-alanine apparent value for wild type enzyme, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 530
-
 D-alanine apparent value for mutant enzyme H244F, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 2000
-
 D-alanine Km above 2000 mM, apparent value for mutant enzyme M261H, at 25°C and pH 8.5 Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.19 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.19 HiTrap chelating affinity chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.19 D-alanine + H2O + O2
-
 Bacillus subtilis pyruvate + NH3 + H2O2
-
 ?
1.4.3.19 D-proline + H2O + O2
-
 Bacillus subtilis ?
-
 ?
1.4.3.19 glycine + H2O + O2
-
 Bacillus subtilis glyoxylate + NH3 + H2O2
-
 ?
1.4.3.19 sarcosine + H2O + O2
-
 Bacillus subtilis glyoxylate + methylamine + H2O2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.19 0.3
-
 D-proline apparent value for mutant enzyme H244N, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 0.3
-
 glycine apparent value for mutant enzyme M261Y, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 0.33
-
 D-alanine kcat above 0.33 1/s, apparent value for mutant enzyme M261H, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 0.38
-
 D-proline apparent value for mutant enzyme M261Y, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 0.47
-
 D-alanine apparent value for mutant enzyme M261Y, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 0.47
-
 sarcosine apparent value for mutant enzyme M261Y, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 0.5
-
 D-proline apparent value for wild type enzyme, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 0.55
-
 glycine apparent value for mutant enzyme M261H, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 0.6
-
 glycine apparent value for wild type enzyme, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 0.6
-
 sarcosine apparent value for wild type enzyme, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 0.62
-
 D-proline apparent value for mutant enzyme M261H, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 0.73
-
 sarcosine apparent value for mutant enzyme M261H, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 0.75
-
 D-alanine apparent value for mutant enzyme H244N, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 0.78
-
 glycine apparent value for mutant enzyme H244F, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 0.92
-
 D-alanine apparent value for wild type enzyme, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 1.08
-
 sarcosine apparent value for mutant enzyme H244F, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 1.08
-
 D-alanine apparent value for mutant enzyme H244Q, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 1.12
-
 sarcosine apparent value for mutant enzyme H244Q, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 1.2
-
 glycine apparent value for mutant enzyme H244Q, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 1.23
-
 D-proline apparent value for mutant enzyme H244Q, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 1.3
-
 glycine apparent value for mutant enzyme H244N, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 1.33
-
 sarcosine apparent value for mutant enzyme H244N, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 1.35
-
 D-proline apparent value for mutant enzyme H244F, at 25°C and pH 8.5 Bacillus subtilis
1.4.3.19 1.5
-
 D-alanine apparent value for mutant enzyme H244F, at 25°C and pH 8.5 Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.19 FAD contains one molecule of non-covalently bound FAD per subunit Bacillus subtilis