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Literature summary extracted from
- Purification, characterization, and substrate specificity of two 2,3-dihydroxybiphenyl 1,2-dioxygenase from Rhodococcus sp. R04, showing their distinct stability at various temperature (2008), Biochimie, 90, 1530-1538.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.39 | Co2+ | 1 mM, 89% inhibition; complete inhibition by 0.1 mM and 1 mM | Rhodococcus pyridinivorans |  |
| 1.13.11.39 | Cu2+ | 0.1 mM caused 92% inhibition; 0.1 mM caused 92% inhibition | Rhodococcus pyridinivorans | |
| 1.13.11.39 | Fe3+ | 0.1 mM, 12% inhibition. 1 mM, 88% inhibition; 0.1 mM, 21% inhibition. 1 mM, 91% inhibition | Rhodococcus pyridinivorans | |
| 1.13.11.39 | H2O2 | 0.05 mM, complete inhibition; 0.05 mM, complete inhibition | Rhodococcus pyridinivorans | |
| 1.13.11.39 | Mn2+ | 1 mM, 87% inhibition | Rhodococcus pyridinivorans | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.39 | 0.0003 | - |
2,3-Dihydroxybiphenyl | 60°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 0.001 | - |
2,3-Dihydroxybiphenyl | 25°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 0.06 | - |
2,3-Dihydroxybiphenyl | 25°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 0.12 | - |
catechol | 60°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 0.4 | - |
catechol | 25°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 0.54 | - |
catechol | 25°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 0.8 | - |
3-chlorocatechol | 60°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 1.1 | - |
3-methylcatechol | 60°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 1.6 | - |
3-chlorocatechol | 25°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 2.1 | - |
3-methylcatechol | 25°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 2.1 | - |
3-chlorocatechol | 25°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 5.6 | - |
4-methylcatechol | 25°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 7.6 | - |
4-methylcatechol | 60°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 11 | - |
4-methylcatechol | 25°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 14.6 | - |
3-methylcatechol | 25°C | Rhodococcus pyridinivorans | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.39 | Co2+ | 0.1 mM, stimulates by 154% | Rhodococcus pyridinivorans | |
| 1.13.11.39 | Fe | 0.8 mol iron per mol subunit of BphC1 | Rhodococcus pyridinivorans | |
| 1.13.11.39 | Fe | 1.0 mol iron per mol subunit of BphC1 | Rhodococcus pyridinivorans | |
| 1.13.11.39 | Mn2+ | 0.1 mM, stimulates by 150% | Rhodococcus pyridinivorans | |
| 1.13.11.39 | Mn2+ | 0.1 mM, stimulates by 164% | Rhodococcus pyridinivorans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.39 | 33000 | - |
6 * 33000, SDS-PAGE | Rhodococcus pyridinivorans |
| 1.13.11.39 | 35000 | - |
6 * 35000, SDS-PAGE | Rhodococcus pyridinivorans |
| 1.13.11.39 | 190000 | - |
gel filtration | Rhodococcus pyridinivorans |
| 1.13.11.39 | 220000 | - |
gel filtration | Rhodococcus pyridinivorans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.39 | Rhodococcus pyridinivorans | Q6QF56 | - |
- |
| 1.13.11.39 | Rhodococcus pyridinivorans | Q6QF57 | fragment | - |
| 1.13.11.39 | Rhodococcus pyridinivorans R04 | Q6QF56 | - |
- |
| 1.13.11.39 | Rhodococcus pyridinivorans R04 | Q6QF57 | fragment | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.39 | - |
Rhodococcus pyridinivorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.39 | 2,3-dihydroxybiphenyl + O2 | - |
Rhodococcus pyridinivorans | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
| 1.13.11.39 | 2,3-dihydroxybiphenyl + O2 | - |
Rhodococcus pyridinivorans R04 | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
| 1.13.11.39 | 3-chlorocatechol + O2 | - |
Rhodococcus pyridinivorans | ? | - |
? | |
| 1.13.11.39 | 3-chlorocatechol + O2 | - |
Rhodococcus pyridinivorans R04 | ? | - |
? | |
| 1.13.11.39 | 3-methylcatechol + O2 | - |
Rhodococcus pyridinivorans | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
| 1.13.11.39 | 3-methylcatechol + O2 | - |
Rhodococcus pyridinivorans R04 | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
| 1.13.11.39 | 4-methylcatechol + O2 | - |
Rhodococcus pyridinivorans | ? | - |
? | |
| 1.13.11.39 | 4-methylcatechol + O2 | - |
Rhodococcus pyridinivorans R04 | ? | - |
? | |
| 1.13.11.39 | catechol + O2 | - |
Rhodococcus pyridinivorans | 2-hydroxymuconate semialdehyde | - |
? | |
| 1.13.11.39 | catechol + O2 | - |
Rhodococcus pyridinivorans R04 | 2-hydroxymuconate semialdehyde | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.11.39 | hexamer | 6 * 35000, SDS-PAGE | Rhodococcus pyridinivorans |
| 1.13.11.39 | hexamer | 6 * 33000, SDS-PAGE | Rhodococcus pyridinivorans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.11.39 | 2,3-dihydroxybiphenyl 1,2-dioxygenase | - |
Rhodococcus pyridinivorans |
| 1.13.11.39 | BphC1 | - |
Rhodococcus pyridinivorans |
| 1.13.11.39 | BphC2 | - |
Rhodococcus pyridinivorans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.39 | 30 | - |
- |
Rhodococcus pyridinivorans |
| 1.13.11.39 | 80 | - |
- |
Rhodococcus pyridinivorans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.39 | 60 | - |
5 min, complete inactivation | Rhodococcus pyridinivorans |
| 1.13.11.39 | 60 | - |
1 h, 38% loss of activity | Rhodococcus pyridinivorans |
| 1.13.11.39 | 70 | - |
1 h, 59% inhibition | Rhodococcus pyridinivorans |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.39 | 1 | - |
4-methylcatechol | 60°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 1 | - |
4-methylcatechol | 25°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 2 | - |
4-methylcatechol | 25°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 3 | - |
3-methylcatechol | 25°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 4 | - |
3-chlorocatechol | 25°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 8 | - |
3-methylcatechol | 25°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 9 | - |
3-chlorocatechol | 25°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 12 | - |
2,3-Dihydroxybiphenyl | 25°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 14 | - |
3-methylcatechol | 60°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 15 | - |
3-chlorocatechol | 60°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 15 | - |
catechol | 25°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 17 | - |
2,3-Dihydroxybiphenyl | 25°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 24 | - |
catechol | 25°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 40 | - |
2,3-Dihydroxybiphenyl | 60°C | Rhodococcus pyridinivorans | |
| 1.13.11.39 | 50 | - |
catechol | 60°C | Rhodococcus pyridinivorans | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.39 | 9 | - |
- |
Rhodococcus pyridinivorans |
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