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Literature summary extracted from

  • Frebortova, J.; Galuszka, P.; Werner, T.; Schmulling, T.; Frebort, I.
    Functional expression and purification of cytokinin dehydrogenase from Arabidopsis thaliana (AtCKX2) in Saccharomyces cerevisiae (2007), Biol. Plant., 51, 673-682.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.99.12 expressed as extracellular protein in Saccharomyces cerevisae Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.99.12 0.0015
-
 N6-(2-isopentenyl)adenine with 2,3-dimethoxy-5-methyl-p-benzoquinone, pH 7.0, 37°C Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.99.12 53300
-
 x * 61000, SDS-PAGE, weak bands also at 75000 Da and100000 Da, x * 53300, calculated from the deduced amino acid sequence Arabidopsis thaliana
1.5.99.12 61000
-
 x * 61000, SDS-PAGE, weak bands also at 75000 Da and100000 Da, x * 53300, calculated from the deduced amino acid sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.99.12 additional information Arabidopsis thaliana irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.12 Arabidopsis thaliana
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.5.99.12 glycoprotein three N-glycosylation sites, glycosylation of the enzyme can explain the difference between theoretical and experimental weight Arabidopsis thaliana

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.99.12 recombinant protein from culture medium Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.99.12 additional information
-
 specific activities given for all possible reactant pairs Arabidopsis thaliana
1.5.99.12 0.0052
-
 N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-p-benzoquinone, pH 7.0, 37°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.12 kinetin + 2,3-dimethoxy-5-methyl-p-benzoquinone low activity Arabidopsis thaliana ?
-
 ?
1.5.99.12 m-topolin + 2,3-dimethoxy-5-methyl-p-benzoquinone very low activity Arabidopsis thaliana ?
-
 ?
1.5.99.12 additional information irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde Arabidopsis thaliana ?
-
 ?
1.5.99.12 additional information AtCKX2 prefers p-quinones and 2,6-dichlorophenol indophenol as electron acceptors, low reactivity with oxygen Arabidopsis thaliana ?
-
 ?
1.5.99.12 additional information no activity with dihydrozeatin Arabidopsis thaliana ?
-
 ?
1.5.99.12 additional information no activity with vitamin K1 as electron acceptor Arabidopsis thaliana ?
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + 1,4-naphthoquinone very effective electron acceptor Arabidopsis thaliana ?
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-p-benzoquinone
-
 Arabidopsis thaliana ?
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + 2,6-dichlorophenol indophenol very effective electron acceptor Arabidopsis thaliana ?
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + CuCl2 low activity Arabidopsis thaliana ?
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + duroquinone low activity Arabidopsis thaliana ?
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + O2 low activity Arabidopsis thaliana ?
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + potassium ferricyanide low activity Arabidopsis thaliana ?
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + vitamin K3 low activity Arabidopsis thaliana ?
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine 9-glucoside + 2,3-dimethoxy-5-methyl-p-benzoquinone low activity Arabidopsis thaliana ?
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine 9-riboside + 2,3-dimethoxy-5-methyl-p-benzoquinone
-
 Arabidopsis thaliana ?
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine 9-riboside-5'-monophosphate + 2,3-dimethoxy-5-methyl-p-benzoquinone low activity Arabidopsis thaliana ?
-
 ?
1.5.99.12 N6-methyl-isopentenyladenine + 2,3-dimethoxy-5-methyl-p-benzoquinone very low activity Arabidopsis thaliana ?
-
 ?
1.5.99.12 o-topolin + 2,3-dimethoxy-5-methyl-p-benzoquinone very low activity Arabidopsis thaliana ?
-
 ?
1.5.99.12 p-topolin + 2,3-dimethoxy-5-methyl-p-benzoquinone very low activity Arabidopsis thaliana ?
-
 ?
1.5.99.12 trans-zeatin + 2,3-dimethoxy-5-methyl-p-benzoquinone very effective substrate Arabidopsis thaliana ?
-
 ?
1.5.99.12 trans-zeatin 9-glucoside + 2,3-dimethoxy-5-methyl-p-benzoquinone low activity Arabidopsis thaliana ?
-
 ?
1.5.99.12 trans-zeatin 9-riboside + 2,3-dimethoxy-5-methyl-p-benzoquinone very effective substrate Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.99.12 More x * 61000, SDS-PAGE, weak bands also at 75000 Da and100000 Da, x * 53300, calculated from the deduced amino acid sequence Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.5.99.12 AtCKX2 seven distinct genes AtCKX1 to AtCKX7, AtCKX2 is subject of the study Arabidopsis thaliana
1.5.99.12 CKX
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.99.12 5.4
-
 N6-(2-isopentenyl)adenine with 2,3-dimethoxy-5-methyl-p-benzoquinone, pH 7.0, 37°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.99.12 7 7.5 with 2,3-dimethoxy-5-methyl-p-benzoquinone Arabidopsis thaliana
1.5.99.12 8
-
 with 2,6-dichlorophenol indophenol Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.12 FAD
-
 Arabidopsis thaliana