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Literature summary extracted from

  • Li, N.; Chu, X.; Wu, L.; Liu, X.; Li, D.
    Functional studies of rat hydroxymethylbilane synthase (2008), Bioorg. Chem., 36, 241-251.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.61 expressed in Escherichia coli strain BL21(DE3) Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.61 D44A specific activity is only about 2% of that of the wild type enzyme Rattus norvegicus
2.5.1.61 E63A reduced activity Rattus norvegicus
2.5.1.61 H78L reduced activity Rattus norvegicus
2.5.1.61 Q200L mutant lacks the dipyrromethane cofactor and completely loses the catalytic activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.61 0.016
-
 porphobilinogen wild type enzyme, at 37°C in Tris-HCl buffer (100 mM, pH 8.0, containing 0.15 mg/ml BSA) Rattus norvegicus
2.5.1.61 0.066
-
 porphobilinogen mutant enzyme E63A, at 37°C in Tris-HCl buffer (100 mM, pH 8.0, containing 0.15 mg/ml BSA) Rattus norvegicus
2.5.1.61 0.24
-
 porphobilinogen mutant enzyme E63A, at 37°C in Tris-HCl buffer (100 mM, pH 8.0, containing 0.15 mg/ml BSA) Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.61 Rattus norvegicus P19356
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.61 Hi-Trap chelating metal affinity column chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.61 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.61 0.001
-
 mutant enzyme D44A, at 37°C Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.5.1.61 -80°C, 50 mM Tris buffer, pH 7.5, 5% glycerol, and 5 mM beta-mercaptoethanol, 1 year, remains stable Rattus norvegicus
2.5.1.61 4°C, 50 mM Tris buffer, pH 7.5, 5% glycerol, and 5 mM beta-mercaptoethanol, 1 week, without significant change of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.61 4 porphobilinogen + H2O
-
 Rattus norvegicus hydroxylmethylbilane + 4 NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.61 EC 4.3.1.8 formerly Rattus norvegicus
2.5.1.61 HMB synthase
-
 Rattus norvegicus
2.5.1.61 PBG deaminase
-
 Rattus norvegicus
2.5.1.61 porphobilinogen deaminase
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.61 60
-
 only a little loss of activity is found even after 1 h preincubation at 60°C Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.61 8
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.61 6 9
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.61 dipyrromethane
-
 Rattus norvegicus