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Literature summary extracted from

  • Hoover, G.J.; Prentice, G.A.; Merrill, A.R.; Shelp, B.J.
    Kinetic mechanism of a recombinant Arabidopsis glyoxylate reductase: studies of initial velocity, dead-end inhibition and product inhibition (2007), Can. J. Bot., 85, 896-902.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.79 expressed in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.79 4-hydroxybutyrate mixed type inhibition with NADPH; mixed type inhibition with succinic semialdehyde Arabidopsis thaliana
1.1.1.79 glycolate mixed type inhibition with glyoxylate; uncompetitive with NADPH Arabidopsis thaliana
1.1.1.79 NADP+ competitive with NADPH; competitive with NADPH when glyoxylate is the fixed substrate; uncompetitive with glyoxylate; uncompetitive with succinic semialdehyde Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.79 0.0045
-
 glyoxylate recombinant protein from Escherichia coli Arabidopsis thaliana
1.1.1.79 0.87
-
 Succinic semialdehyde recombinant protein from Escherichia coli Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.79 Arabidopsis thaliana Q9LSV0 enzyme also displays 4-hydroxybutyrate dehydrogenase (EC1.1.1.61) activity but with less efficiency; L. Heynh
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.79 recombinant protein from Escherichia coli Arabidopsis thaliana

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.79 glycolate + NADP+ = glyoxylate + NADPH + H+ ordered Bi Bi mechanism, complexation of NADPH to the enzyme before glyoxylate or succinic semialdehyde, and release of NADP+ before glycolate or 4-hydroxybutyrate Arabidopsis thaliana
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.79 glyoxylate + NADPH + H+ preferred substrate Arabidopsis thaliana glycolate + NADP+
-
 ir
1.1.1.79 succinic semialdehyde + NADPH + H+
-
 Arabidopsis thaliana 4-hydroxybutyrate + NADP+
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.1.1.79 AtGR1
-
 Arabidopsis thaliana
1.1.1.79 More enzyme also displays 4-hydroxybutyrate dehydrogenase (EC1.1.1.61) activity but with less efficiency Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.79 NADPH
-
 Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.79 0.0031
-
 NADP+ competitive with NADPH Arabidopsis thaliana
1.1.1.79 0.0095
-
 NADP+ competitive with NADPH when glyoxylate is the fixed substrate Arabidopsis thaliana
1.1.1.79 0.125
-
 NADP+ uncompetitive with succinic semialdehyde Arabidopsis thaliana
1.1.1.79 0.147
-
 NADP+ uncompetitive with glyoxylate Arabidopsis thaliana
1.1.1.79 22.1
-
 glycolate uncompetitive with NADPH Arabidopsis thaliana
1.1.1.79 23.7
-
 glycolate mixed type inhibition with glyoxylate Arabidopsis thaliana
1.1.1.79 79.9
-
 4-hydroxybutyrate mixed type inhibition with NADPH Arabidopsis thaliana
1.1.1.79 93.5
-
 4-hydroxybutyrate mixed type inhibition with succinic semialdehyde Arabidopsis thaliana