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Literature summary extracted from

  • Hellgren, M.; Kaiser, C.; de Haij, S.; Norberg, A.; Hoeoeg, J.O.
    A hydrogen-bonding network in mammalian sorbitol dehydrogenase stabilizes the tetrameric state and is essential for the catalytic power (2007), Cell. Mol. Life Sci., 64, 3129-3138.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.14 Y110F mutation in hydrogen onding network, complete loss of activity and destabilization of protein into tetramers, dimers and monomers compared to only tetramers for wild-type Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.14 0.17
-
 D-xylitol wild-type, pH 10.0 Rattus norvegicus
1.1.1.14 0.33
-
 D-sorbitol wild-type, pH 10.0 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.14 40000
-
 plus 80000, 160000 and 300000, mutant Y110F Rattus norvegicus
1.1.1.14 80000
-
 plus 160000, 40000 and 300000, mutant Y110F Rattus norvegicus
1.1.1.14 160000
-
 plus 80000, 40000 and 300000, mutant Y110F Rattus norvegicus
1.1.1.14 300000
-
 above 300000, wild-type and part of mutant Y110F Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.14 Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.14 D-sorbitol + NAD+
-
 Rattus norvegicus D-fructose + NADH + H+
-
 ?
1.1.1.14 D-xylitol + NAD+
-
 Rattus norvegicus ?
-
 ?
1.1.1.14 ethanol + NAD+
-
 Rattus norvegicus acetaldehyde + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.14 dimer plus tetramer and monomer, mutant 110F Rattus norvegicus
1.1.1.14 monomer plus tetramer and monomer, mutant 110F Rattus norvegicus
1.1.1.14 More network of coupled hydrogen bonds holds up the protein interface and tetramer stability Rattus norvegicus
1.1.1.14 tetramer MALDI-TOF, wild-type and part of mutant Y110F Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.14 0.63
-
 D-xylitol wild-type, pH 10.0 Rattus norvegicus
1.1.1.14 0.71
-
 D-sorbitol wild-type, pH 10.0 Rattus norvegicus