EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.14 | Y110F | mutation in hydrogen onding network, complete loss of activity and destabilization of protein into tetramers, dimers and monomers compared to only tetramers for wild-type | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.14 | 0.17 | - |
D-xylitol | wild-type, pH 10.0 | Rattus norvegicus | |
1.1.1.14 | 0.33 | - |
D-sorbitol | wild-type, pH 10.0 | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.14 | 40000 | - |
plus 80000, 160000 and 300000, mutant Y110F | Rattus norvegicus |
1.1.1.14 | 80000 | - |
plus 160000, 40000 and 300000, mutant Y110F | Rattus norvegicus |
1.1.1.14 | 160000 | - |
plus 80000, 40000 and 300000, mutant Y110F | Rattus norvegicus |
1.1.1.14 | 300000 | - |
above 300000, wild-type and part of mutant Y110F | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.14 | Rattus norvegicus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.14 | D-sorbitol + NAD+ | - |
Rattus norvegicus | D-fructose + NADH + H+ | - |
? | |
1.1.1.14 | D-xylitol + NAD+ | - |
Rattus norvegicus | ? | - |
? | |
1.1.1.14 | ethanol + NAD+ | - |
Rattus norvegicus | acetaldehyde + NADH | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.14 | dimer | plus tetramer and monomer, mutant 110F | Rattus norvegicus |
1.1.1.14 | monomer | plus tetramer and monomer, mutant 110F | Rattus norvegicus |
1.1.1.14 | More | network of coupled hydrogen bonds holds up the protein interface and tetramer stability | Rattus norvegicus |
1.1.1.14 | tetramer | MALDI-TOF, wild-type and part of mutant Y110F | Rattus norvegicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.14 | 0.63 | - |
D-xylitol | wild-type, pH 10.0 | Rattus norvegicus | |
1.1.1.14 | 0.71 | - |
D-sorbitol | wild-type, pH 10.0 | Rattus norvegicus |