Literature summary extracted from

Zheng, H.; Chen, J.; Su, L.; Zhao, Y.; Yang, Y.; Zeng, H.Y.; Xu, G.; Yang, S.; Jiang, W.
One-step purification and immobilization of his-tagged GL-7-ACA acylase (2007), Enzyme Microb. Technol., 41, 474-479.

No PubMed abstract available

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.5.1.93	expressed in Escherichia coli BL21 (DE3)/pET28GA01 cells	Pseudomonas sp.

General Stability

EC Number	General Stability	Organism
3.5.1.93	FP-IDA-Ni2+ resins-immobilized GLA is recycled 21times without significant loss of activity	Pseudomonas sp.

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.5.1.93	16000	-	1 * 54000 + 1 * 16000, SDS-PAGE	Pseudomonas sp.
3.5.1.93	54000	-	1 * 54000 + 1 * 16000, SDS-PAGE	Pseudomonas sp.
3.5.1.93	70000	-	SDS-PAGE	Pseudomonas sp.

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.5.1.93	Pseudomonas sp.	O86089	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.5.1.93	TALON affinity resin column chromatography	Pseudomonas sp.

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.5.1.93	glutaryl-7-aminocephalosporanic acid + H2O	-	Pseudomonas sp.	7-aminocephalosporanate + glutarate	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.5.1.93	dimer	1 * 54000 + 1 * 16000, SDS-PAGE	Pseudomonas sp.

Synonyms

EC Number	Synonyms	Comment	Organism
3.5.1.93	cephalosporin acylase	-	Pseudomonas sp.
3.5.1.93	GL-7-ACA acylase	-	Pseudomonas sp.
3.5.1.93	GLA	-	Pseudomonas sp.

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
3.5.1.93	30	-	immobilized GLA shows good stability at temperature up to 30°C	Pseudomonas sp.

pH Stability

EC Number	pH Stability	pH Stability Maximum	Comment	Organism
3.5.1.93	7	11	immobilized GLA shows good stability at pH value below 11.0	Pseudomonas sp.

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Release 2023.1 (January 2023)