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Literature summary extracted from
- Biochemical studies on inositol. V. Purification and properties of the enzyme that cleaves inositol to D-glucuronic acid (1959), J. Biol. Chem., 234, 220-227.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.99.1 | cysteine | best activation system: 1 mM Fe(II) and 2 mM cysteine | Rattus norvegicus |  |
| 1.13.99.1 | quinolinate | 1 mM Fe(II) + 4 mM quinolinate activates to 70% of the Fe(II)/cysteine system, Fe(II) alone causes very little activation, quinolinate gives considerable activation in absence of Fe(II), activation by Fe(II) and quinolinate is very temperature dependent | Rattus norvegicus | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.99.1 | 2-Thenoyltrifluoroacetone | slight | Rattus norvegicus | |
| 1.13.99.1 | 8-hydroxyquinoline | - |
Rattus norvegicus | |
| 1.13.99.1 | ADP | slight | Rattus norvegicus | |
| 1.13.99.1 | Ag+ | - |
Rattus norvegicus | |
| 1.13.99.1 | AMP | - |
Rattus norvegicus | |
| 1.13.99.1 | arsenite | - |
Rattus norvegicus | |
| 1.13.99.1 | ATP | - |
Rattus norvegicus | |
| 1.13.99.1 | azide | - |
Rattus norvegicus | |
| 1.13.99.1 | CTP | - |
Rattus norvegicus | |
| 1.13.99.1 | Cu2+ | - |
Rattus norvegicus | |
| 1.13.99.1 | cyanide | - |
Rattus norvegicus | |
| 1.13.99.1 | FAD | - |
Rattus norvegicus | |
| 1.13.99.1 | Furoylthiofluoroacetone | slight | Rattus norvegicus | |
| 1.13.99.1 | GTP | - |
Rattus norvegicus | |
| 1.13.99.1 | Hg2+ | - |
Rattus norvegicus | |
| 1.13.99.1 | hydroxylamine | - |
Rattus norvegicus | |
| 1.13.99.1 | iodoacetate | - |
Rattus norvegicus | |
| 1.13.99.1 | menadione | - |
Rattus norvegicus | |
| 1.13.99.1 | NAD+ | - |
Rattus norvegicus | |
| 1.13.99.1 | NADP+ | - |
Rattus norvegicus | |
| 1.13.99.1 | o-phenanthroline | slight | Rattus norvegicus | |
| 1.13.99.1 | p-chloromercuribenzoate | - |
Rattus norvegicus | |
| 1.13.99.1 | Phenylmercuric nitrate | - |
Rattus norvegicus | |
| 1.13.99.1 | Quinacrine hydrochloride | slight | Rattus norvegicus | |
| 1.13.99.1 | riboflavin phosphate | - |
Rattus norvegicus | |
| 1.13.99.1 | tetrahydrofolic acid | - |
Rattus norvegicus | |
| 1.13.99.1 | Uridine diphosphoglucose | - |
Rattus norvegicus | |
| 1.13.99.1 | UTP | - |
Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.99.1 | 22.1 | - |
Inositol | - |
Rattus norvegicus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.99.1 | Fe2+ | best activation system | Rattus norvegicus | |
| 1.13.99.1 | Fe2+ | 1 mM Fe(II) + 4 mM quinolinate activate to 70% of the Fe(II)/cysteine system, Fe(II) alone causes very little activation, quinolinate gives considerable activation in absence of Fe(II), activation by Fe(II) and quinolinate is very temperature dependent | Rattus norvegicus | |
| 1.13.99.1 | Fe2+ | 1 mM Fe(II) and 2 mM cysteine | Rattus norvegicus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.99.1 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.99.1 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.13.99.1 | kidney | - |
Rattus norvegicus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.13.99.1 | -20°C, extensive loss of activity after 1 or 2 days | Rattus norvegicus |
| 1.13.99.1 | 0°C, 12 h, extensive loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.99.1 | myo-inositol + O2 | highly specific for myo-inositol | Rattus norvegicus | D-glucuronate + H2O | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.99.1 | 35 | - |
assay at | Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.99.1 | 7.22 | - |
Inositol | - |
Rattus norvegicus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.99.1 | 6.8 | 7.2 | - |
Rattus norvegicus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.99.1 | 6.5 | 7.4 | sharp decrease of activity below pH 6.5 and above pH 7.4 | Rattus norvegicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.99.1 | flavin | 5. 6 mMol per mol of enzyme | Rattus norvegicus | |
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