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Literature summary extracted from

  • Cai, X.; Lorraine Fuller, A.; McDougald, L.R.; Tan, X.; Cai, J.; Wang, F.; Sacchettini, J.C.; Zhu, G.
    Biochemical characterization of enoyl reductase involved in Type II fatty acid synthesis in the intestinal coccidium Eimeria tenella (Phylum Apicomplexa) (2007), FEMS Microbiol. Lett., 272, 238-244.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.9 expressed in Escherichia coli Eimeria tenella

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.9 crotonoyl-CoA the addition of short-chain crotonoyl-CoA actually inhibits the oxidation of NADH by recombinant ENR in a dose-dependent manner Eimeria tenella
1.3.1.9 triclosan
-
Eimeria tenella

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.9 0.08832
-
NADH recombinant enzyme, in the absence of enoyl-CoA as scosubstrate, in 0.1 mM Tris-HCl buffer (pH 7.2) Eimeria tenella

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Eimeria tenella A3R4P1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.9 amylose-resin-based affinity chromatography Eimeria tenella

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 crotonyl-CoA + NADH + H+
-
Eimeria tenella butyryl-CoA + NAD+
-
?
1.3.1.9 dodecenoyl-CoA + NADH + H+
-
Eimeria tenella dodecanoyl-CoA + NAD+
-
?
1.3.1.9 additional information the recombinant ENR expressed in bacteria is only able to oxidize NADH, but unable to transfer the electron to enoyl-CoA, possibly due to the inappropriate folding of ENR expressed in bacteria Eimeria tenella ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.9 enoyl reductase the enzyme is involved in Type II fatty acid synthesis Eimeria tenella
1.3.1.9 ENR
-
Eimeria tenella

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.9 NADH
-
Eimeria tenella

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.3.1.9 0.00145
-
recombinant enzyme, in 0.1 mM Tris-HCl buffer (pH 7.2) Eimeria tenella triclosan