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Literature summary extracted from

  • Demuez, M.; Cournac, L.; Guerrini, O.; Soucaille, P.; Girbal, L.
    Complete activity profile of Clostridium acetobutylicum [FeFe]-hydrogenase and kinetic parameters for endogenous redox partners (2007), FEMS Microbiol. Lett., 275, 113-121.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.7.2 expressed in Escherichia coli Clostridium acetobutylicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.7.2 0.000033
-
 H2 using ferredoxin CAC0587 as electron carrier, at 35°C Clostridium acetobutylicum
1.12.7.2 0.000088
-
 H2 using flavodoxin CAC0587 as electron carrier, at 35°C Clostridium acetobutylicum
1.12.7.2 0.016
-
 H2 using methyl viologen as electron carrier, at 35°C Clostridium acetobutylicum

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Clostridium acetobutylicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2
-
 Clostridium acetobutylicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H2 + oxidized ferredoxin CAC0587 ferredoxin CAC0587 is the standard major ferredoxin Clostridium acetobutylicum H+ + reduced ferredoxin CAC0587
-
 r
1.12.7.2 H2 + oxidized flavodoxin CAC0587 flavodoxin CAC0587 is the standard major flavodoxin Clostridium acetobutylicum H+ + reduced flavodoxin CAC0587
-
 r
1.12.7.2 H2 + oxidized methyl viologen
-
 Clostridium acetobutylicum H+ + reduced methyl viologen
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.12.7.2 HydA1
-
 Clostridium acetobutylicum
1.12.7.2 [FeFe]-hydrogenase
-
 Clostridium acetobutylicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.12.7.2 133
-
 H2 using ferredoxin CAC0587 as electron carrier, at 35°C Clostridium acetobutylicum
1.12.7.2 454
-
 H2 using methyl viologen as electron carrier, at 35°C Clostridium acetobutylicum
1.12.7.2 483
-
 H2 using flavodoxin CAC0587 as electron carrier, at 35°C Clostridium acetobutylicum